TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes

Qun He, Hai Yan Huang, You You Zhang, Xi Li, Shu Wen Qian, Qi Qun Tang

Research output: Contribution to journalArticle

Abstract

TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX), one component of induction cocktails routinely used in adipocyte differentiation. DEX repressed the transcription of TAZ by direct binding of the glucocorticoid receptor (GR) to the GR binding element in its promoter. More importantly, overexpression of TAZ inhibited adipogenesis and promoted the trans-differentiation of preadipocytes into osteocytes. This establishes a new functional interaction between DEX and TAZ that contributes to the mechanism of adipogenesis.

Original languageEnglish (US)
Pages (from-to)573-577
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number3
DOIs
StatePublished - Mar 16 2012
Externally publishedYes

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Keywords

  • Adipocyte differentiation
  • Dexamethasone
  • TAZ
  • Trans-differentiation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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