TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes

Qun He; Hai Yan Huang; You You Zhang; Xi Li; Shu Wen Qian; Qi Qun Tang

doi:10.1016/j.bbrc.2012.02.074

TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes

Qun He, Hai Yan Huang, You You Zhang, Xi Li, Shu Wen Qian, Qi Qun Tang

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX), one component of induction cocktails routinely used in adipocyte differentiation. DEX repressed the transcription of TAZ by direct binding of the glucocorticoid receptor (GR) to the GR binding element in its promoter. More importantly, overexpression of TAZ inhibited adipogenesis and promoted the trans-differentiation of preadipocytes into osteocytes. This establishes a new functional interaction between DEX and TAZ that contributes to the mechanism of adipogenesis.

Original language	English (US)
Pages (from-to)	573-577
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	419
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2012.02.074
State	Published - Mar 16 2012
Externally published	Yes

Keywords

Adipocyte differentiation
Dexamethasone
TAZ
Trans-differentiation

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Molecular Biology

Access to Document

10.1016/j.bbrc.2012.02.074

Cite this

@article{c26ca3373ae94f369607949b37ae62f9,

title = "TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes",

abstract = "TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX), one component of induction cocktails routinely used in adipocyte differentiation. DEX repressed the transcription of TAZ by direct binding of the glucocorticoid receptor (GR) to the GR binding element in its promoter. More importantly, overexpression of TAZ inhibited adipogenesis and promoted the trans-differentiation of preadipocytes into osteocytes. This establishes a new functional interaction between DEX and TAZ that contributes to the mechanism of adipogenesis.",

keywords = "Adipocyte differentiation, Dexamethasone, TAZ, Trans-differentiation",

author = "Qun He and Huang, {Hai Yan} and Zhang, {You You} and Xi Li and Qian, {Shu Wen} and Tang, {Qi Qun}",

year = "2012",

month = mar,

day = "16",

doi = "10.1016/j.bbrc.2012.02.074",

language = "English (US)",

volume = "419",

pages = "573--577",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes

AU - He, Qun

AU - Huang, Hai Yan

AU - Zhang, You You

AU - Li, Xi

AU - Qian, Shu Wen

AU - Tang, Qi Qun

PY - 2012/3/16

Y1 - 2012/3/16

N2 - TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX), one component of induction cocktails routinely used in adipocyte differentiation. DEX repressed the transcription of TAZ by direct binding of the glucocorticoid receptor (GR) to the GR binding element in its promoter. More importantly, overexpression of TAZ inhibited adipogenesis and promoted the trans-differentiation of preadipocytes into osteocytes. This establishes a new functional interaction between DEX and TAZ that contributes to the mechanism of adipogenesis.

AB - TAZ (transcriptional co-activator with PDZ binding motif) is a transcriptional modulator of mesenchymal stem cell differentiation. We have found that TAZ was expressed in postconfluent 3T3-L1 preadipocytes and downregulated during differentiation. Downregulation of TAZ was specifically mediated by dexamethasone (DEX), one component of induction cocktails routinely used in adipocyte differentiation. DEX repressed the transcription of TAZ by direct binding of the glucocorticoid receptor (GR) to the GR binding element in its promoter. More importantly, overexpression of TAZ inhibited adipogenesis and promoted the trans-differentiation of preadipocytes into osteocytes. This establishes a new functional interaction between DEX and TAZ that contributes to the mechanism of adipogenesis.

KW - Adipocyte differentiation

KW - Dexamethasone

KW - TAZ

KW - Trans-differentiation

UR - http://www.scopus.com/inward/record.url?scp=84862800398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862800398&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2012.02.074

DO - 10.1016/j.bbrc.2012.02.074

M3 - Article

C2 - 22374070

AN - SCOPUS:84862800398

SN - 0006-291X

VL - 419

SP - 573

EP - 577

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

TAZ is downregulated by dexamethasone during the differentiation of 3T3-L1 preadipocytes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this