Taurodeoxycholate stimulates intestinal cell proliferation and protects against apoptotic cell death through activation of NF-κB

Alexander Toledo, Jon Yamaguchi, Jian Ying Wang, Barbara L. Bass, Douglas J. Turner, Eric D. Strauch

Research output: Contribution to journalArticle

Abstract

We hypothesized that the NF-κB pathway would be operative in the proliferative effect of bile salts on enterocytes. To determine this, we studied the effect of the bile salt taurodeoxycholate on cultured rat enterocyte proliferation and apoptosis and examined the role of NF-κB activation in these growth regulatory processes. Intestinal epithelial cells were grown for 6 days with or without taurodeoxycholate. Proliferation was measured. The cells were exposed to a known apoptotic stimulus, TNF-α and cyclohexamide. Apoptosis was quantified using cell number and the TUNEL stain. NF-κB activation was determined by an electrophoretic mobility shift assay. NF-κB activation was inhibited by an IκB superrepressor. Taurodeoxycholate stimulated cell proliferation (P

Original languageEnglish (US)
Pages (from-to)1664-1671
Number of pages8
JournalDigestive Diseases and Sciences
Volume49
Issue number10
DOIs
StatePublished - Oct 2004
Externally publishedYes

Fingerprint

Taurodeoxycholic Acid
Cell Death
Enterocytes
Cell Proliferation
Bile Acids and Salts
Apoptosis
In Situ Nick-End Labeling
Electrophoretic Mobility Shift Assay
Coloring Agents
Cell Count
Epithelial Cells
Growth

Keywords

  • apoptosis
  • bile salts
  • enterocyte
  • NF-κB
  • proliferation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Taurodeoxycholate stimulates intestinal cell proliferation and protects against apoptotic cell death through activation of NF-κB. / Toledo, Alexander; Yamaguchi, Jon; Wang, Jian Ying; Bass, Barbara L.; Turner, Douglas J.; Strauch, Eric D.

In: Digestive Diseases and Sciences, Vol. 49, No. 10, 10.2004, p. 1664-1671.

Research output: Contribution to journalArticle

Toledo, Alexander ; Yamaguchi, Jon ; Wang, Jian Ying ; Bass, Barbara L. ; Turner, Douglas J. ; Strauch, Eric D. / Taurodeoxycholate stimulates intestinal cell proliferation and protects against apoptotic cell death through activation of NF-κB. In: Digestive Diseases and Sciences. 2004 ; Vol. 49, No. 10. pp. 1664-1671.
@article{ef00f0b1bc594167b1e571c469dbfce1,
title = "Taurodeoxycholate stimulates intestinal cell proliferation and protects against apoptotic cell death through activation of NF-κB",
abstract = "We hypothesized that the NF-κB pathway would be operative in the proliferative effect of bile salts on enterocytes. To determine this, we studied the effect of the bile salt taurodeoxycholate on cultured rat enterocyte proliferation and apoptosis and examined the role of NF-κB activation in these growth regulatory processes. Intestinal epithelial cells were grown for 6 days with or without taurodeoxycholate. Proliferation was measured. The cells were exposed to a known apoptotic stimulus, TNF-α and cyclohexamide. Apoptosis was quantified using cell number and the TUNEL stain. NF-κB activation was determined by an electrophoretic mobility shift assay. NF-κB activation was inhibited by an IκB superrepressor. Taurodeoxycholate stimulated cell proliferation (P",
keywords = "apoptosis, bile salts, enterocyte, NF-κB, proliferation",
author = "Alexander Toledo and Jon Yamaguchi and Wang, {Jian Ying} and Bass, {Barbara L.} and Turner, {Douglas J.} and Strauch, {Eric D.}",
year = "2004",
month = "10",
doi = "10.1023/B:DDAS.0000043383.96077.99",
language = "English (US)",
volume = "49",
pages = "1664--1671",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Plenum Publishers",
number = "10",

}

TY - JOUR

T1 - Taurodeoxycholate stimulates intestinal cell proliferation and protects against apoptotic cell death through activation of NF-κB

AU - Toledo, Alexander

AU - Yamaguchi, Jon

AU - Wang, Jian Ying

AU - Bass, Barbara L.

AU - Turner, Douglas J.

AU - Strauch, Eric D.

PY - 2004/10

Y1 - 2004/10

N2 - We hypothesized that the NF-κB pathway would be operative in the proliferative effect of bile salts on enterocytes. To determine this, we studied the effect of the bile salt taurodeoxycholate on cultured rat enterocyte proliferation and apoptosis and examined the role of NF-κB activation in these growth regulatory processes. Intestinal epithelial cells were grown for 6 days with or without taurodeoxycholate. Proliferation was measured. The cells were exposed to a known apoptotic stimulus, TNF-α and cyclohexamide. Apoptosis was quantified using cell number and the TUNEL stain. NF-κB activation was determined by an electrophoretic mobility shift assay. NF-κB activation was inhibited by an IκB superrepressor. Taurodeoxycholate stimulated cell proliferation (P

AB - We hypothesized that the NF-κB pathway would be operative in the proliferative effect of bile salts on enterocytes. To determine this, we studied the effect of the bile salt taurodeoxycholate on cultured rat enterocyte proliferation and apoptosis and examined the role of NF-κB activation in these growth regulatory processes. Intestinal epithelial cells were grown for 6 days with or without taurodeoxycholate. Proliferation was measured. The cells were exposed to a known apoptotic stimulus, TNF-α and cyclohexamide. Apoptosis was quantified using cell number and the TUNEL stain. NF-κB activation was determined by an electrophoretic mobility shift assay. NF-κB activation was inhibited by an IκB superrepressor. Taurodeoxycholate stimulated cell proliferation (P

KW - apoptosis

KW - bile salts

KW - enterocyte

KW - NF-κB

KW - proliferation

UR - http://www.scopus.com/inward/record.url?scp=4944267762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4944267762&partnerID=8YFLogxK

U2 - 10.1023/B:DDAS.0000043383.96077.99

DO - 10.1023/B:DDAS.0000043383.96077.99

M3 - Article

C2 - 15573924

AN - SCOPUS:4944267762

VL - 49

SP - 1664

EP - 1671

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 10

ER -