Targeting single neuronal networks for gene expression and cell labeling in vivo

James H. Marshel, Takuma Mori, Kristina J Nielsen, Edward M. Callaway

Research output: Contribution to journalArticle

Abstract

To understand fine-scale structure and function of single mammalian neuronal networks, we developed and validated a strategy to genetically target and trace monosynaptic inputs to a single neuron in vitro and in vivo. The strategy independently targets a neuron and its presynaptic network for specific gene expression and fine-scale labeling, using single-cell electroporation of DNA to target infection and monosynaptic retrograde spread of a genetically modifiable rabies virus. The technique is highly reliable, with transsynaptic labeling occurring in every electroporated neuron infected by the virus. Targeting single neocortical neuronal networks in vivo, we found clusters of both spiny and aspiny neurons surrounding the electroporated neuron in each case, in addition to intricately labeled distal cortical and subcortical inputs. This technique, broadly applicable for probing and manipulating single neuronal networks with single-cell resolution in vivo, may help shed new light on fundamental mechanisms underlying circuit development and information processing by neuronal networks throughout the brain.

Original languageEnglish (US)
Pages (from-to)562-574
Number of pages13
JournalNeuron
Volume67
Issue number4
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Gene Expression
Neurons
Rabies virus
Electroporation
Automatic Data Processing
Viruses
DNA
Brain
Infection

Keywords

  • Molneuro
  • Sysneuro

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Targeting single neuronal networks for gene expression and cell labeling in vivo. / Marshel, James H.; Mori, Takuma; Nielsen, Kristina J; Callaway, Edward M.

In: Neuron, Vol. 67, No. 4, 2010, p. 562-574.

Research output: Contribution to journalArticle

Marshel, James H. ; Mori, Takuma ; Nielsen, Kristina J ; Callaway, Edward M. / Targeting single neuronal networks for gene expression and cell labeling in vivo. In: Neuron. 2010 ; Vol. 67, No. 4. pp. 562-574.
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