Targeting of PKA to glutamate receptors through a MAGUK-AKAP complex

Marcie Colledge, Rebecca A. Dean, Gregory K. Scott, Lorene K. Langeberg, Richard L. Huganir, John D. Scott

Research output: Contribution to journalArticlepeer-review

Abstract

Compartmentalization of glutamate receptors with the signaling enzymes that regulate their activity supports synaptic transmission. Two classes of binding proteins organize these complexes: the MAGUK proteins that cluster glutamate receptors and AKAPs that anchor kinases and phosphatases. In this report, we demonstrate that glutamate receptors and PKA are recruited into a macromolecular signaling complex through direct interaction between the MAGUK proteins, PSD-95 and SAP97, and AKAP79/150. The SH3 and GK regions of the MAGUKs mediate binding to the AKAP. Cell-based studies indicate that phosphorylation of AMPA receptors is enhanced by a SAP97-AKAP79 complex that directs PKA to GluR1 via a PDZ domain interaction. As AMPA receptor phosphorylation is implicated in regulating synaptic plasticity, these data suggest that a MAGUK-AKAP complex may be centrally involved.

Original languageEnglish (US)
Pages (from-to)107-119
Number of pages13
JournalNeuron
Volume27
Issue number1
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'Targeting of PKA to glutamate receptors through a MAGUK-AKAP complex'. Together they form a unique fingerprint.

Cite this