TY - JOUR
T1 - Targeting mitochondrial oxidative metabolism in melanoma causes metabolic compensation through glucose and glutamine utilization
AU - Lim, Ji Hong
AU - Luo, Chi
AU - Vazquez, Francisca
AU - Puigserver, Pere
PY - 2014/7/1
Y1 - 2014/7/1
N2 - Metabolic targets offer attractive opportunities for cancer therapy. However, their targeting may activate alternative metabolic pathways that can still support tumor growth. A subset of human melanomas relies on PGC1a-dependent mitochondrial oxidative metabolism to maintain growth and survival. Herein, we showthat loss of viability caused by suppression of PGC1a in these melanomas is rescued by induction of glycolysis. Suppression of PGC1a elevates reactive oxygen species levels decreasing hypoxia-inducible factor-1a (HIF1a) hydroxylation that, in turn, increases its protein stability. HIF1a reprograms melanomas to become highly glycolytic and dependent on this pathway for survival. Dual suppression of PGC1a and HIF1a causes energetic deficits and loss of viability that are partially compensated by glutamine utilization. Notably, triple suppression of PGC1a, HIF1a, and glutamine utilization results in complete blockage of tumor growth. These results show that due to high metabolic and bioenergetic flexibility, complete treatment of melanomas will require combinatorial therapy that targets multiple metabolic components.
AB - Metabolic targets offer attractive opportunities for cancer therapy. However, their targeting may activate alternative metabolic pathways that can still support tumor growth. A subset of human melanomas relies on PGC1a-dependent mitochondrial oxidative metabolism to maintain growth and survival. Herein, we showthat loss of viability caused by suppression of PGC1a in these melanomas is rescued by induction of glycolysis. Suppression of PGC1a elevates reactive oxygen species levels decreasing hypoxia-inducible factor-1a (HIF1a) hydroxylation that, in turn, increases its protein stability. HIF1a reprograms melanomas to become highly glycolytic and dependent on this pathway for survival. Dual suppression of PGC1a and HIF1a causes energetic deficits and loss of viability that are partially compensated by glutamine utilization. Notably, triple suppression of PGC1a, HIF1a, and glutamine utilization results in complete blockage of tumor growth. These results show that due to high metabolic and bioenergetic flexibility, complete treatment of melanomas will require combinatorial therapy that targets multiple metabolic components.
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U2 - 10.1158/0008-5472.CAN-13-2893-T
DO - 10.1158/0008-5472.CAN-13-2893-T
M3 - Article
C2 - 24812272
AN - SCOPUS:84903976031
VL - 74
SP - 3535
EP - 3545
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 13
ER -