Potential bone marrow donors are screened to ensure the safety of both the donor and recipient. At our institution, potential donors with abnormal peripheral blood cell counts, a personal history of malignancy, or age >60 years are evaluated to ensure that they are viable candidates for donation. Evaluation of the marrow includes morphologic, flow cytometric, and cytogenetic studies. A total of 122 potential donors were screened between the years of 2001and 2011, encompassing approximately 10% of all donors. Of the screened potential donors, the mean age was 59 years and there were 59 men and 63 women. The donors were screened because of age >60 years (n= 33), anemia (n= 22), cytopenias other than anemia (n= 27), elevated peripheral blood counts without a concurrent cytopenia (n= 20), elevated peripheral blood counts with a concurrent cytopenia (n= 10), history of malignancy (n= 4), abnormal peripheral blood differential (n= 3), prior graft failure (n= 1), history of treatment with chemotherapy (n= 1), and body habitus (n= 1). Marrow abnormalities were detected in 9% (11 of 122) of donors. These donors were screened because of anemia (5 of 22, 23%), age >60 years (2 of 33, 6%), history of malignancy (2 of 4, 50%), elevated peripheral blood counts (1 of 20, 5%), and body habitus (1 of 1, 100%). Abnormalities included plasma cell dyscrasia (n= 3), abnormal marrow cellularity (n= 3), clonal cytogenetic abnormalities (n= 2), low-grade myelodysplastic syndrome (1), a mutated JAK2 V617F allele (n= 1), and monoclonal B cell lymphocytosis (n= 1). Our experience indicates that extended screening of potential donors identifies a significant number of donors with previously undiagnosed marrow abnormalities.
- Flow cytometry
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