Targeted gene deletion of prolyl hydroxylase domain protein 3 triggers angiogenesis and preserves cardiac function by stabilizing hypoxia inducible factor 1 alpha following myocardial infarction

Babatunde Oriowo, Mahesh Thirunavukkarasu, Vaithinathan Selvaraju, Ram Sudheer Adluri, Lijun Zhan, Kotaro Takeda, Guo Hua Fong, Juan Sanchez, Nilanjana Maulik

Research output: Contribution to journalArticle

Abstract

The key oxygen sensing molecules, Prolyl-hydroxylase domain 1-3 enzymes (PHD1-3), regulate hypoxia-inducible factor (HIF) under hypoxia. In the settings of cardiomyopathy and ischemia-reperfusion injury, PHD3 expression is elevated, resulting in decreased HIF activation. The role of PHD3 in myocardial injury is poorly understood. Hence, we aimed to determine the effects of PHD3 deletion in mice on HIF-1α and other related pathways following myocardial infarction (MI). Left coronary artery (LAD) in both wild type and prolyl hydroxylase 3 knock out (PHD3-/-) mice was ligated to induce myocardial infarction. Electrophoretic mobility shift analysis showed significant increase in DNA-binding activity of HIF-1α in PHD3-/- mice as compared to wild type (WT) mice post MI. The PHD3-/-MI group also showed decreased fibrosis. Seven days after MI, enhanced capillary / arteriolar density was observed compared to WTMI group. PHD3-/- mice subjected to MI also showed improved cardiac functions (Ejection fraction and Fractional shortening), as assessed by echocardiogram, compared to WT. Western blot analysis showed increased VEGF, Ang-1 & Bcl-2 expression in PHD3-/-MI group. In conclusion, ablation of the PHD3 gene resulted in increased angiogenesis and cardiac function after infarction thereby offering a potential target for pharmacological management of ischemic myocardial disease.

Original languageEnglish (US)
Pages (from-to)1305-1310
Number of pages6
JournalCurrent Pharmaceutical Design
Volume20
Issue number9
DOIs
StatePublished - 2014
Externally publishedYes

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Prolyl Hydroxylases
Hypoxia-Inducible Factor 1
Gene Deletion
Myocardial Infarction
Cardiomyopathies
Protein Domains
Reperfusion Injury
Knockout Mice
Infarction
Vascular Endothelial Growth Factor A
Coronary Vessels
Fibrosis
Western Blotting
Pharmacology
Oxygen
DNA
Wounds and Injuries
Enzymes

Keywords

  • Angiogenesis
  • Bcl-2
  • HIF
  • Myocardial infarction
  • Prolyl hydroxylase
  • VEGF

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Medicine(all)

Cite this

Targeted gene deletion of prolyl hydroxylase domain protein 3 triggers angiogenesis and preserves cardiac function by stabilizing hypoxia inducible factor 1 alpha following myocardial infarction. / Oriowo, Babatunde; Thirunavukkarasu, Mahesh; Selvaraju, Vaithinathan; Adluri, Ram Sudheer; Zhan, Lijun; Takeda, Kotaro; Fong, Guo Hua; Sanchez, Juan; Maulik, Nilanjana.

In: Current Pharmaceutical Design, Vol. 20, No. 9, 2014, p. 1305-1310.

Research output: Contribution to journalArticle

Oriowo, Babatunde ; Thirunavukkarasu, Mahesh ; Selvaraju, Vaithinathan ; Adluri, Ram Sudheer ; Zhan, Lijun ; Takeda, Kotaro ; Fong, Guo Hua ; Sanchez, Juan ; Maulik, Nilanjana. / Targeted gene deletion of prolyl hydroxylase domain protein 3 triggers angiogenesis and preserves cardiac function by stabilizing hypoxia inducible factor 1 alpha following myocardial infarction. In: Current Pharmaceutical Design. 2014 ; Vol. 20, No. 9. pp. 1305-1310.
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AU - Adluri, Ram Sudheer

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