Targeted delivery of immunomicrospheres in vivo

Vu L. Truong, Jerry R. Williams, James E K Hildreth, Kam W. Leong

    Research output: Contribution to journalArticle

    Abstract

    The feasibility of targeted delivery of bioactive agents using microspheres made of enzymatically degradable gelatin/chondroitin sulfate was investigated. The microspheres, synthesized by complex coacervation under mild aqueous conditions, exhibited high loading level for a variety of agents. Monoclonal anti-bodies against P-glycoprotein (Pgp), lymphocyte function associated antigen-1 (LFA-1), and thrombomodulin (TM) were attached to the microsphere surface using avidin and biotin as the linkage system. Anti-Pgp mAb-coated microsphere encapsulated with photofrin II destroyed Pgp-positive KB-V-1 cells 24 times more efficient as compared to the free drug in vitro. The binding and destruction of KB-V-1 cells were cell tar-get selective and antibody dependent. Electron microscopy studies showed that colloidal gold-encapsulated microspheres coated with anti-Pgp mAb were internalized by KB-V-1 cells within 4 h after binding. The target selectability of this immunomicrosphere delivery system was also demonstrated in vivo. 90Yttrium-labeled microspheres were targeted to the pulmonary vascular endothelial cells of mice using anti-TM mAb, and to the leukocytes using anti-LFA-1 mAb. A total of 67.5% of anti-TM mAb coated microspheres (injected dose per gram wet tissue weight) was localized to the lung within 1 h after injection, while 20.2% of anti-LFA-1 mAb-coated microspheres was associated with the blood compartment.

    Original languageEnglish (US)
    Pages (from-to)166-174
    Number of pages9
    JournalDrug Delivery
    Volume2
    Issue number3-4
    DOIs
    StatePublished - 1995

    ASJC Scopus subject areas

    • Pharmaceutical Science
    • Pharmacology

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  • Cite this

    Truong, V. L., Williams, J. R., Hildreth, J. E. K., & Leong, K. W. (1995). Targeted delivery of immunomicrospheres in vivo. Drug Delivery, 2(3-4), 166-174. https://doi.org/10.3109/10717549509031366