Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1): Biochemical and morphological consequences in adult mice

Harriet Baker, Diana M. Cummings, Steven D. Munger, Joyce W. Margolis, Linda Franzen, Randall R Reed, Frank L. Margolis

Research output: Contribution to journalArticle

Abstract

The olfactory cyclic nucleotide-gated channel subunit 1 (OCNC1) is required for signal transduction in olfactory receptor cells. To further investigate the role of this channel in the olfactory system, the biochemical and morphological consequences of targeted disruption of OCNC1 were investigated in adult mice. Null as compared to wild-type mice had smaller olfactory bulbs, suggesting compromised development of the central target of the receptor cells. Ectopic olfactory marker protein (OMP)-stained fibers localized to the external plexiform layer reflected the relative immaturity of the olfactory bulb in the null mice. The olfactory epithelium of the knock-out mouse was thinner and showed lower expression of olfactory marker protein and growth-associated protein 43, indicating decreases in both generation and maturation of receptor cells. Tyrosine hydroxylase (TH) expression in the olfactory bulb, examined as a reflection of afferent activity, was reduced in the majority of periglomerular neurons but retained in atypical or 'necklace' glomeruli localized to posterior aspects of the olfactory bulb. Double label studies demonstrated that the remaining TH- immunostained neurons received their innervation from a subset of receptor cells previously shown to express a phosphodiesterase that differs from that found in most receptor cells. These data indicate that expression of OCNC1 is required for normal development of the olfactory epithelium and olfactory bulb. The robust expression of TH in some periglomerular cells in the OCNC1- null mice suggests that receptor cells innervating these glomeruli may use an alternate signal transduction pathway.

Original languageEnglish (US)
Pages (from-to)9313-9321
Number of pages9
JournalJournal of Neuroscience
Volume19
Issue number21
StatePublished - Nov 1 1999

Fingerprint

Cyclic Nucleotide-Gated Cation Channels
Olfactory Bulb
Olfactory Marker Protein
Tyrosine 3-Monooxygenase
Olfactory Mucosa
Signal Transduction
Olfactory Receptor Neurons
GAP-43 Protein
Neurons
Phosphoric Diester Hydrolases
Knockout Mice

Keywords

  • Atypical glomeruli
  • Necklace glomeruli
  • Olfactory marker protein
  • PAX6
  • Phosphodiesterase
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Baker, H., Cummings, D. M., Munger, S. D., Margolis, J. W., Franzen, L., Reed, R. R., & Margolis, F. L. (1999). Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1): Biochemical and morphological consequences in adult mice. Journal of Neuroscience, 19(21), 9313-9321.

Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1) : Biochemical and morphological consequences in adult mice. / Baker, Harriet; Cummings, Diana M.; Munger, Steven D.; Margolis, Joyce W.; Franzen, Linda; Reed, Randall R; Margolis, Frank L.

In: Journal of Neuroscience, Vol. 19, No. 21, 01.11.1999, p. 9313-9321.

Research output: Contribution to journalArticle

Baker, H, Cummings, DM, Munger, SD, Margolis, JW, Franzen, L, Reed, RR & Margolis, FL 1999, 'Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1): Biochemical and morphological consequences in adult mice', Journal of Neuroscience, vol. 19, no. 21, pp. 9313-9321.
Baker, Harriet ; Cummings, Diana M. ; Munger, Steven D. ; Margolis, Joyce W. ; Franzen, Linda ; Reed, Randall R ; Margolis, Frank L. / Targeted deletion of a cyclic nucleotide-gated channel subunit (OCNC1) : Biochemical and morphological consequences in adult mice. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 21. pp. 9313-9321.
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