Targeted cross-linking of the human β-globin gene in living cells mediated by a triple helix forming oligonucleotide

Kazi Abdus Shahid, Alokes Majumdar, Rowshon Alam, Su Ting Liu, Jean Y. Kuan, Xuifen Sui, Bernard Cuenoud, Peter M. Glazer, Paul S. Miller, Michael M. Seidman

Research output: Contribution to journalArticlepeer-review

Abstract

Triple helix forming oligonucleotides (TFOs) may have utility as gene targeting reagents for "in situ" gene therapy of genetic disorders. Triplex formation is challenged by negative charge repulsion between third strand and duplex phosphates, and destabilizing positive charge repulsion between adjacent protonated cytosines within pyrimidine motif third strands. Here we describe the synthesis of TFOs designed to target a site in the human β-globin gene, which is the locus for mutations that underlie the β-globinopathies, including sickle cell anemia. The target is an uninterrupted polypurineipolypyrimidine sequence, containing four adjacent cytosines, next to a psoralen cross-link site. Pyrimidine motif TFOs that contained four adjacent cytosines or 5-methylcytosines did not form stable triplexes at physiological pH, despite the introduction of otherwise stabilizing base and sugar analogues. We synthesized a series of pso-TFOs containing 2′-O-methyl (OMe) and 2′-O-aminoethoxy substitutions (AE), as well as 8-oxoadenine (A8) and 2′-O-methylpseudoisocytidine (P) as neutral cytosine replacements. Thermal stability measurements indicated that TFOs with A8 did not meet criteria established in previous work. However, TFOs with P did form triplexes with appropriate Tm and kON values. A pso-TFO with AE and P residues was sufficiently active to permit the determination of targeting in living cells by direct measurement of cross-link formation at the target site. Our results validate the modification format described in our previous studies and indicate that P substitutions are an effective solution to the problem of targeting genomic sequences containing adjacent cytosines.

Original languageEnglish (US)
Pages (from-to)1970-1978
Number of pages9
JournalBiochemistry
Volume45
Issue number6
DOIs
StatePublished - Feb 14 2006

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Targeted cross-linking of the human β-globin gene in living cells mediated by a triple helix forming oligonucleotide'. Together they form a unique fingerprint.

Cite this