Targeted ablation of beta cells in the embryonic zebrafish pancreas using E. coli nitroreductase

Harshan Pisharath, Jerry M. Rhee, Michelle A. Swanson, Steven D. Leach, Michael J. Parsons

Research output: Contribution to journalArticlepeer-review

Abstract

In order to generate a zebrafish model of β cell regeneration, we have expressed an Escherichia coli gene called nfsB in the β cells of embryonic zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme, which can convert prodrugs such as metronidazole (Met) to cytotoxins. By fusing nfsB to mCherry, we can simultaneously render β cells susceptible to prodrug and visualize Met dependent cell ablation. We show that the neighboring α and δ cells are unaffected by prodrug treatment and that ablation is β cell specific. Following drug removal and 36 h of recovery, β cells regenerate. Using ptf1a morphants, it is clear that this β cell recovery occurs independently of the presence of the exocrine pancreas. Also, by using photoconvertible Kaede to cell lineage trace and BrdU incorporation to label proliferation, we investigate mechanisms for β regeneration. Therefore, we have developed a unique resource for the study of β cell regeneration in a living vertebrate organism, which will provide the opportunity to conduct large-scale screens for pharmacological and genetic modifiers of β cell regeneration.

Original languageEnglish (US)
Pages (from-to)218-229
Number of pages12
JournalMechanisms of Development
Volume124
Issue number3
DOIs
StatePublished - Mar 2007

Keywords

  • Cell lineage tracing
  • Diabetes
  • Differentiation
  • Disease model
  • Prodrug
  • Regeneration

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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