Target DNA chromatinization modulates nicking by L1 endonuclease

Gregory J. Cost, Amit Golding, Mark S. Schlissel, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

L1 elements are human transposons which replicate via an RNA intermediate. At least 15% of the human genome is composed of L1 sequence. An important initial step in the transposition reaction is nicking of the genomic DNA by L1 endonuclease (L1 EN). In vivo much of the genome exists in the form of chromatin or is undergoing biochemical transactions such as transcription, replication or repair, which may alter the accessibility of the L1 transposition machinery to DNA. To investigate this possibility we have examined the effect of substrate chromatinization on the ability of L1 EN to nick DNA. We find that DNA incorporated into nucleosomes is generally refractory to nicking by L1 EN. Interestingly, nicking of a minority of DNA sequences is enhanced when included in chromatin. Thus, dynamic epigenetic factors such as chromatinization are likely to influence the relatively permanent placement of L1 and other retroelements in the human genome.

Original languageEnglish (US)
Pages (from-to)573-577
Number of pages5
JournalNucleic Acids Research
Volume29
Issue number2
StatePublished - Jan 15 2001

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Endonucleases
Human Genome
Chromatin
Long Interspersed Nucleotide Elements
Single-Stranded DNA Breaks
Retroelements
Nucleosomes
Deoxyribonuclease I
DNA
Epigenomics
Genome
RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Cost, G. J., Golding, A., Schlissel, M. S., & Boeke, J. D. (2001). Target DNA chromatinization modulates nicking by L1 endonuclease. Nucleic Acids Research, 29(2), 573-577.

Target DNA chromatinization modulates nicking by L1 endonuclease. / Cost, Gregory J.; Golding, Amit; Schlissel, Mark S.; Boeke, Jef D.

In: Nucleic Acids Research, Vol. 29, No. 2, 15.01.2001, p. 573-577.

Research output: Contribution to journalArticle

Cost, GJ, Golding, A, Schlissel, MS & Boeke, JD 2001, 'Target DNA chromatinization modulates nicking by L1 endonuclease', Nucleic Acids Research, vol. 29, no. 2, pp. 573-577.
Cost GJ, Golding A, Schlissel MS, Boeke JD. Target DNA chromatinization modulates nicking by L1 endonuclease. Nucleic Acids Research. 2001 Jan 15;29(2):573-577.
Cost, Gregory J. ; Golding, Amit ; Schlissel, Mark S. ; Boeke, Jef D. / Target DNA chromatinization modulates nicking by L1 endonuclease. In: Nucleic Acids Research. 2001 ; Vol. 29, No. 2. pp. 573-577.
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