Target cells for an immunosuppressive cytokine, glycosylation-inhibiting factor

Katsuji Sugie, Takafumi Tomura, Kenji Takakura, Tetsu Kawano, Masaru Taniguchi, Howard M. Grey, Kimishige Ishizaka

Research output: Contribution to journalArticle

Abstract

Receptors for bioactive glycosylation-inhibiting factor (GIF) were demonstrated using a bioactive mutant of recombinant human (rh) GIF, which is comparable to the suppressor T (T(s)) cell-derived bioactive GIF in its affinity for the receptors on helper T (T(h)) hybridoma cells. Both naive T and B cells in normal mouse spleen lacked GIF receptors. However, presentation of specific antigen to naive T cells resulted in the expression of the receptors on activated T cells. Furthermore, activation of small resting B cells with F(ab')2 fragments of anti-mouse IgM plus IL-4, lipopolysaccharide (LPS) plus IL-4 or LPS plus dextran sulfate induced the expression of the receptors within 48 h of B cell stimulation. It was also found that NK T cells freshly isolated from mouse spleen, but not conventional NK cells, expressed receptors for GIF. CD4+ and CD4- subpopulations of NK T cells showed a similar binding capability. Mature dendritic cells derived from bone marrow did not bear the receptors. The dissociation constant (K(d)) Of the interaction between the bioactive rhGIF mutant and the high-affinity receptors was 10-100 pM, whereas inactive wild-type rhGIF failed to bind to the receptors. A bioactive derivative of rhGIF suppressed both IgG1 and IgE synthesis by purified B cells activated by LPS and IL-4, indicating that the binding of bioactive GIF to its receptors on activated B cells results in suppression of their differentiation.

Original languageEnglish (US)
Pages (from-to)1149-1156
Number of pages8
JournalInternational Immunology
Volume11
Issue number7
DOIs
StatePublished - Jul 29 1999

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Keywords

  • Cytokine receptor
  • Ig

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Sugie, K., Tomura, T., Takakura, K., Kawano, T., Taniguchi, M., Grey, H. M., & Ishizaka, K. (1999). Target cells for an immunosuppressive cytokine, glycosylation-inhibiting factor. International Immunology, 11(7), 1149-1156. https://doi.org/10.1093/intimm/11.7.1149