Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice

Sooyeon Yoo, David Cha, Soohyun Kim, Lizhi Jiang, Patrick Cooke, Mobolanie Adebesin, Andrew Wolfe, Ryan Riddle, Susan Aja, Seth Blackshaw

Research output: Contribution to journalArticlepeer-review

Abstract

Tanycytes are radial glial cells located in the mediobasal hypothalamus. Recent studies have proposed that tanycytes play an important role in hypothalamic control of energy homeostasis, although this has not been directly tested. Here, we report the phenotype of mice in which tanycytes of the arcuate nucleus and median eminence were conditionally ablated in adult mice. Although the cerebrospinal fluid-hypothalamic barrier was rendered more permeable following tanycyte ablation, neither the blood-hypothalamic barrier nor leptin-induced pSTAT3 activation in hypothalamic parenchyma were affected. We observed a significant increase in visceral fat distribution accompanying insulin insensitivity in male mice, without significant effect on either body weight or food intake. A high-fat diet tended to accelerate overall body weight gain in tanycyte-ablated mice, but the development of visceral adiposity and insulin insensitivity was comparable to wildtype. Thermoneutral housing exacerbated fat accumulation and produced a shift away from fat oxidation in tanycyte-ablated mice. These results clarify the extent to which tanycytes regulate energy balance, and demonstrate a role for tanycytes in regulating fat metabolism.

Original languageEnglish (US)
Pages (from-to)1987-2000
Number of pages14
JournalGlia
Volume68
Issue number10
DOIs
StatePublished - Oct 1 2020

Keywords

  • adipose
  • blood–brain barrier
  • energy homeostasis
  • hypothalamus
  • insulin insensitivity
  • metabolism
  • radial glia
  • tanycytes
  • thyroid

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

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