Tangential migration of neuronal precursors of glutamatergic neurons in the adult mammalian brain

Gerald J. Sun, Yi Zhou, Ryan P. Stadel, Jonathan Moss, Jing Hui A. Yong, Shiori Ito, Nicholas K. Kawasaki, Alexander T. Phan, Justin H. Oh, Nikhil Modak, Randall R. Reed, Nicolas Toni, Hongjun Song, Guo Li Ming

Research output: Contribution to journalArticle

Abstract

In a classic model of mammalian brain formation, precursors of principal glutamatergic neurons migrate radially along radial glia fibers whereas GABAergic interneuron precursors migrate tangentially. These migration modes have significant implications for brain function. Here we used clonal lineage tracing of active radial glia-like neural stem cells in the adult mouse dentate gyrus and made the surprising discovery that proliferating neuronal precursors of glutamatergic granule neurons exhibit significant tangential migration along blood vessels, followed by limited radial migration. Genetic birthdating and morphological and molecular analyses pinpointed the neuroblast stage as the main developmental window when tangential migration occurs. We also developed a partial "wholemount" dentate gyrus preparation and observed a dense plexus of capillaries, with which only neuroblasts, among the entire population of progenitors, are directly associated. Together, these results provide insight into neuronal migration in the adult mammalian nervous system.

Original languageEnglish (US)
Pages (from-to)9484-9489
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number30
DOIs
StatePublished - Jul 28 2015

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Keywords

  • Adult neurogenesis
  • Hippocampus
  • Lineage tracing
  • Migration
  • Vascular niche

ASJC Scopus subject areas

  • General

Cite this

Sun, G. J., Zhou, Y., Stadel, R. P., Moss, J., Yong, J. H. A., Ito, S., Kawasaki, N. K., Phan, A. T., Oh, J. H., Modak, N., Reed, R. R., Toni, N., Song, H., & Ming, G. L. (2015). Tangential migration of neuronal precursors of glutamatergic neurons in the adult mammalian brain. Proceedings of the National Academy of Sciences of the United States of America, 112(30), 9484-9489. https://doi.org/10.1073/pnas.1508545112