TY - JOUR
T1 - Tandem time-of-flight (TOF/TOF) mass spectrometry and the curved-field reflectron
AU - Cotter, Robert J.
AU - Griffith, Wendell
AU - Jelinek, Christine
N1 - Funding Information:
This work was carried out at the Middle Atlantic Mass Spectrometry Laboratory at the Johns Hopkins School of Medicine and supported in part by a contract (N01 HV28180) to J. VanEyk from the National Heart, Lung and Blood institute, a grant (GM/RR64402) from NIH to R.J. Cotter and a grant (U54 RR020839) to Jef Boeke.
PY - 2007/8/1
Y1 - 2007/8/1
N2 - The curved-field reflectron (CFR), when used as the second mass analyzer in a tandem time-of-flight mass spectrometer, provides a design that enables the use of very high energy collision-induced dissociation (CID). Specifically, this is because the wide energy bandwidth of the CFR obviates the need for floating the collision region to decelerate the precursor ions and subsequently reaccelerating product ions to enable reflectron focusing. Here we describe the evolution of tandem instruments based on the CFR, from its introduction in 1993 to the current commercial TOF2 mass spectrometer from Shimadzu Corporation, and briefly review the history of TOF/TOF instruments. A number of applications are also described. One is the characterization of a C-terminal cleavage of cystatin C that appears to be associated with patients with remitting relapse multiple sclerosis (RRMS). Both surface-enhanced laser desorption/ionization (SELDI) and MALDI were used on a high performance TOF instrument operating in the MS and MS/MS modes. Tandem TOF mass spectrometry has also been used to determine the acetylation sites on histones and on the enzyme, histone acetyl transferase (HAT), responsible for the modification. Acetylation has been determined quantitatively for multiple sites on histone H3 and H4 using a deuteroacetylation method. For a number of closely spaced sites on the histone tail regions, MS/MS enables us to then determine both the order and distribution of acetylation.
AB - The curved-field reflectron (CFR), when used as the second mass analyzer in a tandem time-of-flight mass spectrometer, provides a design that enables the use of very high energy collision-induced dissociation (CID). Specifically, this is because the wide energy bandwidth of the CFR obviates the need for floating the collision region to decelerate the precursor ions and subsequently reaccelerating product ions to enable reflectron focusing. Here we describe the evolution of tandem instruments based on the CFR, from its introduction in 1993 to the current commercial TOF2 mass spectrometer from Shimadzu Corporation, and briefly review the history of TOF/TOF instruments. A number of applications are also described. One is the characterization of a C-terminal cleavage of cystatin C that appears to be associated with patients with remitting relapse multiple sclerosis (RRMS). Both surface-enhanced laser desorption/ionization (SELDI) and MALDI were used on a high performance TOF instrument operating in the MS and MS/MS modes. Tandem TOF mass spectrometry has also been used to determine the acetylation sites on histones and on the enzyme, histone acetyl transferase (HAT), responsible for the modification. Acetylation has been determined quantitatively for multiple sites on histone H3 and H4 using a deuteroacetylation method. For a number of closely spaced sites on the histone tail regions, MS/MS enables us to then determine both the order and distribution of acetylation.
KW - Acetylation
KW - Curved-field reflectron
KW - Cystatin
KW - Histone acetyl transferase (HAT)
KW - Histones
KW - Matrix-assisted laser desorption/ionization (MALDI)
KW - Multiple sclerosis
KW - Surface-enhanced laser desorption/ionization (SELDI)
KW - Tandem mass spectrometry
KW - Time-of-flight
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U2 - 10.1016/j.jchromb.2007.01.009
DO - 10.1016/j.jchromb.2007.01.009
M3 - Review article
C2 - 17258517
AN - SCOPUS:34447567531
VL - 855
SP - 2
EP - 13
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1 SPEC. ISS.
ER -