Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions

Francesco J. Conti, Sue J. Monkley, Malcolm R. Wood, David R. Critchley, Ulrich Mueller

Research output: Contribution to journalArticle

Abstract

Talin 1 and 2 connect integrins to the actin cytoskeleton and regulate the affinity of integrins for ligands. In skeletal muscle, talin 1 regulates the stability of myotendinous junctions (MTJs), but the function of talin 2 in skeletal muscle is not known. Here we show that MTJ integrity is affected in talin 2-deficient mice. Concomitant ablation of talin 1 and 2 leads to defects in myoblast fusion and sarcomere assembly, resembling defects in muscle lacking β1 integrins. Talin 1/2-deficient myoblasts express functionally active β1 integrins, suggesting that defects in muscle development are not primarily caused by defects in ligand binding, but rather by disruptions of the interaction of integrins with the cytoskeleton. Consistent with this finding, assembly of integrin adhesion complexes is perturbed in the remaining muscle fibers of talin 1/2-deficient mice. We conclude that talin 1 and 2 are crucial for skeletal muscle development, where they regulate myoblast fusion, sarcomere assembly and the maintenance of MTJs.

Original languageEnglish (US)
Pages (from-to)3597-3606
Number of pages10
JournalDevelopment
Volume136
Issue number21
DOIs
StatePublished - Nov 1 2009
Externally publishedYes

Fingerprint

Talin
Sarcomeres
Myoblasts
Maintenance
Integrins
Skeletal Muscle
Muscle Development
Ligands
Muscles
Cytoskeleton
Actin Cytoskeleton

Keywords

  • Dystrophin
  • Integrin
  • Mice
  • Muscular dystrophy
  • Talin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions. / Conti, Francesco J.; Monkley, Sue J.; Wood, Malcolm R.; Critchley, David R.; Mueller, Ulrich.

In: Development, Vol. 136, No. 21, 01.11.2009, p. 3597-3606.

Research output: Contribution to journalArticle

Conti, Francesco J. ; Monkley, Sue J. ; Wood, Malcolm R. ; Critchley, David R. ; Mueller, Ulrich. / Talin 1 and 2 are required for myoblast fusion, sarcomere assembly and the maintenance of myotendinous junctions. In: Development. 2009 ; Vol. 136, No. 21. pp. 3597-3606.
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