Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages

Karen K. Klyczek, J. Alfred Bonilla, Deborah Jacobs-Sera, Tamarah L. Adair, Patricia Afram, Katherine G. Allen, Megan L. Archambault, Rahat M. Aziz, Filippa G. Bagnasco, Sarah L. Ball, Natalie A. Barrett, Robert C. Benjamin, Christopher J. Blasi, Katherine Borst, Mary A. Braun, Haley Broomell, Conner B. Brown, Zachary S. Brynell, Ashley B. Bue, Sydney O. BurkeWilliam Casazza, Julia A. Cautela, Kevin Chen, Nitish S. Chimalakonda, Dylan Chudoff, Jade A. Connor, Trevor S. Cross, Kyra N. Curtis, Jessica A. Dahlke, Bethany M. Deaton, Sarah J. Degroote, Danielle M. Denigris, Katherine C. Deruff, Milan Dolan, David Dunbar, Marisa S. Egan, Daniel R. Evans, Abby K. Fahnestock, Amal Farooq, Garrett Finn, Christopher R. Fratus, Bobby L. Gaffney, Rebecca A. Garlena, Kelly E. Garrigan, Bryan C. Gibbon, Michael A. Goedde, Carlos A. Guerrero Bustamante, Melinda Harrison, Megan C. Hartwell, Emily L. Heckman, Jennifer Huang, Lee E. Hughes, Kathryn M. Hyduchak, Aswathi E. Jacob, Machika Kaku, Allen W. Karstens, Margaret A. Kenna, Susheel Khetarpal, Rodney A. King, Amanda L. Kobokovich, Hannah Kolev, Sai A. Konde, Elizabeth Kriese, Morgan E. Lamey, Carter N. Lantz, Jonathan S. Lapin, Temiloluwa O. Lawson, In Young Lee, Scott M. Lee, Julia Y. Lee-Soety, Emily M. Lehmann, Shawn C. London, A. Javier Lopez, Kelly C. Lynch, Catherine M. Mageeney, Tetyana Martynyuk, Kevin J. Mathew, Travis N. Mavrich, Christopher M. McDaniel, Hannah McDonald, C. Joel McManus, Jessica E. Medrano, Francis E. Mele, Jennifer E. Menninger, Sierra N. Miller, Josephine E. Minick, Courtney T. Nabua, Caroline K. Napoli, Martha Nkangabwa, Elizabeth A. Oates, Cassandra T. Ott, Sarah K. Pellerino, William J. Pinamont, Ross T. Pirnie, Marie C. Pizzorno, Emilee J. Plautz, Welkin H. Pope, Katelyn M. Pruett, Gabbi Rickstrew, Patrick A. Rimple, Claire A. Rinehart, Kayla M. Robinson, Victoria A. Rose, Daniel A. Russell, Amelia M. Schick, Julia Schlossman, Victoria M. Schneider, Chloe A. Sells, Jeremy W. Sieker, Morgan P. Silva, Marissa M. Silvi, Stephanie E. Simon, Amanda K. Staples, Isabelle L. Steed, Emily L. Stowe, Noah A. Stueven, Porter T. Swartz, Emma A. Sweet, Abigail T. Sweetman, Corrina Tender, Katrina Terry, Chrystal Thomas, Daniel S. Thomas, Allison R. Thompson, Lorianna Vanderveen, Rohan Varma, Hannah L. Vaught, Quynh D. Vo, Zachary T. Vonberg, Vassie C. Ware, Yasmene M. Warrad, Kaitlyn E. Wathen, Jonathan L. Weinstein, Jacqueline F. Wyper, Jakob R. Yankauskas, Christine Zhang, Graham F. Hatfull

Research output: Contribution to journalArticlepeer-review

Abstract

The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45–68%, compared to 63.4% for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate.

Original languageEnglish (US)
Article numbere0180517
JournalPloS one
Volume12
Issue number7
DOIs
StatePublished - Jul 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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