Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages

Karen K. Klyczek, J. Alfred Bonilla, Deborah Jacobs-Sera, Tamarah L. Adair, Patricia Afram, Katherine G. Allen, Megan L. Archambault, Rahat M. Aziz, Filippa G. Bagnasco, Sarah L. Ball, Natalie A. Barrett, Robert C. Benjamin, Christopher J. Blasi, Katherine Borst, Mary A. Braun, Haley Broomell, Conner B. Brown, Zachary S. Brynell, Ashley B. Bue, Sydney O. BurkeWilliam Casazza, Julia A. Cautela, Kevin Chen, Nitish S. Chimalakonda, Dylan Chudoff, Jade A. Connor, Trevor S. Cross, Kyra N. Curtis, Jessica A. Dahlke, Bethany M. Deaton, Sarah J. Degroote, Danielle M. Denigris, Katherine C. Deruff, Milan Dolan, David Dunbar, Marisa S. Egan, Daniel R. Evans, Abby K. Fahnestock, Amal Farooq, Garrett Finn, Christopher R. Fratus, Bobby L. Gaffney, Rebecca A. Garlena, Kelly E. Garrigan, Bryan C. Gibbon, Michael A. Goedde, Carlos A. Guerrero Bustamante, Melinda Harrison, Megan C. Hartwell, Emily L. Heckman, Jennifer Huang, Lee E. Hughes, Kathryn M. Hyduchak, Aswathi E. Jacob, Machika Kaku, Allen W. Karstens, Margaret A. Kenna, Susheel Khetarpal, Rodney A. King, Amanda Kobokovich, Hannah Kolev, Sai A. Konde, Elizabeth Kriese, Morgan E. Lamey, Carter N. Lantz, Jonathan S. Lapin, Temiloluwa O. Lawson, In Young Lee, Scott M. Lee, Julia Y. Lee-Soety, Emily M. Lehmann, Shawn C. London, A. Javier Lopez, Kelly C. Lynch, Catherine M. Mageeney, Tetyana Martynyuk, Kevin J. Mathew, Travis N. Mavrich, Christopher M. McDaniel, Hannah McDonald, C. Joel McManus, Jessica E. Medrano, Francis E. Mele, Jennifer E. Menninger, Sierra N. Miller, Josephine E. Minick, Courtney T. Nabua, Caroline K. Napoli, Martha Nkangabwa, Elizabeth A. Oates, Cassandra T. Ott, Sarah K. Pellerino, William J. Pinamont, Ross T. Pirnie, Marie C. Pizzorno, Emilee J. Plautz, Welkin H. Pope, Katelyn M. Pruett, Gabbi Rickstrew, Patrick A. Rimple, Claire A. Rinehart, Kayla M. Robinson, Victoria A. Rose, Daniel A. Russell, Amelia M. Schick, Julia Schlossman, Victoria M. Schneider, Chloe A. Sells, Jeremy W. Sieker, Morgan P. Silva, Marissa M. Silvi, Stephanie E. Simon, Amanda K. Staples, Isabelle L. Steed, Emily L. Stowe, Noah A. Stueven, Porter T. Swartz, Emma A. Sweet, Abigail T. Sweetman, Corrina Tender, Katrina Terry, Chrystal Thomas, Daniel S. Thomas, Allison R. Thompson, Lorianna Vanderveen, Rohan Varma, Hannah L. Vaught, Quynh D. Vo, Zachary T. Vonberg, Vassie C. Ware, Yasmene M. Warrad, Kaitlyn E. Wathen, Jonathan L. Weinstein, Jacqueline F. Wyper, Jakob R. Yankauskas, Christine Zhang, Graham F. Hatfull

Research output: Contribution to journalArticle

Abstract

The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45–68%, compared to 63.4% for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate.

Original languageEnglish (US)
Article numbere0180517
JournalPLoS One
Volume12
Issue number7
DOIs
StatePublished - Jul 1 2017
Externally publishedYes

Fingerprint

Arthrobacter
Bacteriophages
bacteriophages
genomics
Genes
genome
Genome
Jasminum
Galaxies
Lysogeny
lysogeny
Genome Size
Geographic Locations
Mosaicism
Actinobacteria
Base Composition
Ports and harbors
virion
Virion
Nucleotides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Klyczek, K. K., Bonilla, J. A., Jacobs-Sera, D., Adair, T. L., Afram, P., Allen, K. G., ... Hatfull, G. F. (2017). Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages. PLoS One, 12(7), [e0180517]. https://doi.org/10.1371/journal.pone.0180517

Tales of diversity : Genomic and morphological characteristics of forty-six Arthrobacter phages. / Klyczek, Karen K.; Bonilla, J. Alfred; Jacobs-Sera, Deborah; Adair, Tamarah L.; Afram, Patricia; Allen, Katherine G.; Archambault, Megan L.; Aziz, Rahat M.; Bagnasco, Filippa G.; Ball, Sarah L.; Barrett, Natalie A.; Benjamin, Robert C.; Blasi, Christopher J.; Borst, Katherine; Braun, Mary A.; Broomell, Haley; Brown, Conner B.; Brynell, Zachary S.; Bue, Ashley B.; Burke, Sydney O.; Casazza, William; Cautela, Julia A.; Chen, Kevin; Chimalakonda, Nitish S.; Chudoff, Dylan; Connor, Jade A.; Cross, Trevor S.; Curtis, Kyra N.; Dahlke, Jessica A.; Deaton, Bethany M.; Degroote, Sarah J.; Denigris, Danielle M.; Deruff, Katherine C.; Dolan, Milan; Dunbar, David; Egan, Marisa S.; Evans, Daniel R.; Fahnestock, Abby K.; Farooq, Amal; Finn, Garrett; Fratus, Christopher R.; Gaffney, Bobby L.; Garlena, Rebecca A.; Garrigan, Kelly E.; Gibbon, Bryan C.; Goedde, Michael A.; Guerrero Bustamante, Carlos A.; Harrison, Melinda; Hartwell, Megan C.; Heckman, Emily L.; Huang, Jennifer; Hughes, Lee E.; Hyduchak, Kathryn M.; Jacob, Aswathi E.; Kaku, Machika; Karstens, Allen W.; Kenna, Margaret A.; Khetarpal, Susheel; King, Rodney A.; Kobokovich, Amanda; Kolev, Hannah; Konde, Sai A.; Kriese, Elizabeth; Lamey, Morgan E.; Lantz, Carter N.; Lapin, Jonathan S.; Lawson, Temiloluwa O.; Lee, In Young; Lee, Scott M.; Lee-Soety, Julia Y.; Lehmann, Emily M.; London, Shawn C.; Lopez, A. Javier; Lynch, Kelly C.; Mageeney, Catherine M.; Martynyuk, Tetyana; Mathew, Kevin J.; Mavrich, Travis N.; McDaniel, Christopher M.; McDonald, Hannah; McManus, C. Joel; Medrano, Jessica E.; Mele, Francis E.; Menninger, Jennifer E.; Miller, Sierra N.; Minick, Josephine E.; Nabua, Courtney T.; Napoli, Caroline K.; Nkangabwa, Martha; Oates, Elizabeth A.; Ott, Cassandra T.; Pellerino, Sarah K.; Pinamont, William J.; Pirnie, Ross T.; Pizzorno, Marie C.; Plautz, Emilee J.; Pope, Welkin H.; Pruett, Katelyn M.; Rickstrew, Gabbi; Rimple, Patrick A.; Rinehart, Claire A.; Robinson, Kayla M.; Rose, Victoria A.; Russell, Daniel A.; Schick, Amelia M.; Schlossman, Julia; Schneider, Victoria M.; Sells, Chloe A.; Sieker, Jeremy W.; Silva, Morgan P.; Silvi, Marissa M.; Simon, Stephanie E.; Staples, Amanda K.; Steed, Isabelle L.; Stowe, Emily L.; Stueven, Noah A.; Swartz, Porter T.; Sweet, Emma A.; Sweetman, Abigail T.; Tender, Corrina; Terry, Katrina; Thomas, Chrystal; Thomas, Daniel S.; Thompson, Allison R.; Vanderveen, Lorianna; Varma, Rohan; Vaught, Hannah L.; Vo, Quynh D.; Vonberg, Zachary T.; Ware, Vassie C.; Warrad, Yasmene M.; Wathen, Kaitlyn E.; Weinstein, Jonathan L.; Wyper, Jacqueline F.; Yankauskas, Jakob R.; Zhang, Christine; Hatfull, Graham F.

In: PLoS One, Vol. 12, No. 7, e0180517, 01.07.2017.

Research output: Contribution to journalArticle

Klyczek, KK, Bonilla, JA, Jacobs-Sera, D, Adair, TL, Afram, P, Allen, KG, Archambault, ML, Aziz, RM, Bagnasco, FG, Ball, SL, Barrett, NA, Benjamin, RC, Blasi, CJ, Borst, K, Braun, MA, Broomell, H, Brown, CB, Brynell, ZS, Bue, AB, Burke, SO, Casazza, W, Cautela, JA, Chen, K, Chimalakonda, NS, Chudoff, D, Connor, JA, Cross, TS, Curtis, KN, Dahlke, JA, Deaton, BM, Degroote, SJ, Denigris, DM, Deruff, KC, Dolan, M, Dunbar, D, Egan, MS, Evans, DR, Fahnestock, AK, Farooq, A, Finn, G, Fratus, CR, Gaffney, BL, Garlena, RA, Garrigan, KE, Gibbon, BC, Goedde, MA, Guerrero Bustamante, CA, Harrison, M, Hartwell, MC, Heckman, EL, Huang, J, Hughes, LE, Hyduchak, KM, Jacob, AE, Kaku, M, Karstens, AW, Kenna, MA, Khetarpal, S, King, RA, Kobokovich, A, Kolev, H, Konde, SA, Kriese, E, Lamey, ME, Lantz, CN, Lapin, JS, Lawson, TO, Lee, IY, Lee, SM, Lee-Soety, JY, Lehmann, EM, London, SC, Lopez, AJ, Lynch, KC, Mageeney, CM, Martynyuk, T, Mathew, KJ, Mavrich, TN, McDaniel, CM, McDonald, H, McManus, CJ, Medrano, JE, Mele, FE, Menninger, JE, Miller, SN, Minick, JE, Nabua, CT, Napoli, CK, Nkangabwa, M, Oates, EA, Ott, CT, Pellerino, SK, Pinamont, WJ, Pirnie, RT, Pizzorno, MC, Plautz, EJ, Pope, WH, Pruett, KM, Rickstrew, G, Rimple, PA, Rinehart, CA, Robinson, KM, Rose, VA, Russell, DA, Schick, AM, Schlossman, J, Schneider, VM, Sells, CA, Sieker, JW, Silva, MP, Silvi, MM, Simon, SE, Staples, AK, Steed, IL, Stowe, EL, Stueven, NA, Swartz, PT, Sweet, EA, Sweetman, AT, Tender, C, Terry, K, Thomas, C, Thomas, DS, Thompson, AR, Vanderveen, L, Varma, R, Vaught, HL, Vo, QD, Vonberg, ZT, Ware, VC, Warrad, YM, Wathen, KE, Weinstein, JL, Wyper, JF, Yankauskas, JR, Zhang, C & Hatfull, GF 2017, 'Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages', PLoS One, vol. 12, no. 7, e0180517. https://doi.org/10.1371/journal.pone.0180517
Klyczek KK, Bonilla JA, Jacobs-Sera D, Adair TL, Afram P, Allen KG et al. Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages. PLoS One. 2017 Jul 1;12(7). e0180517. https://doi.org/10.1371/journal.pone.0180517
Klyczek, Karen K. ; Bonilla, J. Alfred ; Jacobs-Sera, Deborah ; Adair, Tamarah L. ; Afram, Patricia ; Allen, Katherine G. ; Archambault, Megan L. ; Aziz, Rahat M. ; Bagnasco, Filippa G. ; Ball, Sarah L. ; Barrett, Natalie A. ; Benjamin, Robert C. ; Blasi, Christopher J. ; Borst, Katherine ; Braun, Mary A. ; Broomell, Haley ; Brown, Conner B. ; Brynell, Zachary S. ; Bue, Ashley B. ; Burke, Sydney O. ; Casazza, William ; Cautela, Julia A. ; Chen, Kevin ; Chimalakonda, Nitish S. ; Chudoff, Dylan ; Connor, Jade A. ; Cross, Trevor S. ; Curtis, Kyra N. ; Dahlke, Jessica A. ; Deaton, Bethany M. ; Degroote, Sarah J. ; Denigris, Danielle M. ; Deruff, Katherine C. ; Dolan, Milan ; Dunbar, David ; Egan, Marisa S. ; Evans, Daniel R. ; Fahnestock, Abby K. ; Farooq, Amal ; Finn, Garrett ; Fratus, Christopher R. ; Gaffney, Bobby L. ; Garlena, Rebecca A. ; Garrigan, Kelly E. ; Gibbon, Bryan C. ; Goedde, Michael A. ; Guerrero Bustamante, Carlos A. ; Harrison, Melinda ; Hartwell, Megan C. ; Heckman, Emily L. ; Huang, Jennifer ; Hughes, Lee E. ; Hyduchak, Kathryn M. ; Jacob, Aswathi E. ; Kaku, Machika ; Karstens, Allen W. ; Kenna, Margaret A. ; Khetarpal, Susheel ; King, Rodney A. ; Kobokovich, Amanda ; Kolev, Hannah ; Konde, Sai A. ; Kriese, Elizabeth ; Lamey, Morgan E. ; Lantz, Carter N. ; Lapin, Jonathan S. ; Lawson, Temiloluwa O. ; Lee, In Young ; Lee, Scott M. ; Lee-Soety, Julia Y. ; Lehmann, Emily M. ; London, Shawn C. ; Lopez, A. Javier ; Lynch, Kelly C. ; Mageeney, Catherine M. ; Martynyuk, Tetyana ; Mathew, Kevin J. ; Mavrich, Travis N. ; McDaniel, Christopher M. ; McDonald, Hannah ; McManus, C. Joel ; Medrano, Jessica E. ; Mele, Francis E. ; Menninger, Jennifer E. ; Miller, Sierra N. ; Minick, Josephine E. ; Nabua, Courtney T. ; Napoli, Caroline K. ; Nkangabwa, Martha ; Oates, Elizabeth A. ; Ott, Cassandra T. ; Pellerino, Sarah K. ; Pinamont, William J. ; Pirnie, Ross T. ; Pizzorno, Marie C. ; Plautz, Emilee J. ; Pope, Welkin H. ; Pruett, Katelyn M. ; Rickstrew, Gabbi ; Rimple, Patrick A. ; Rinehart, Claire A. ; Robinson, Kayla M. ; Rose, Victoria A. ; Russell, Daniel A. ; Schick, Amelia M. ; Schlossman, Julia ; Schneider, Victoria M. ; Sells, Chloe A. ; Sieker, Jeremy W. ; Silva, Morgan P. ; Silvi, Marissa M. ; Simon, Stephanie E. ; Staples, Amanda K. ; Steed, Isabelle L. ; Stowe, Emily L. ; Stueven, Noah A. ; Swartz, Porter T. ; Sweet, Emma A. ; Sweetman, Abigail T. ; Tender, Corrina ; Terry, Katrina ; Thomas, Chrystal ; Thomas, Daniel S. ; Thompson, Allison R. ; Vanderveen, Lorianna ; Varma, Rohan ; Vaught, Hannah L. ; Vo, Quynh D. ; Vonberg, Zachary T. ; Ware, Vassie C. ; Warrad, Yasmene M. ; Wathen, Kaitlyn E. ; Weinstein, Jonathan L. ; Wyper, Jacqueline F. ; Yankauskas, Jakob R. ; Zhang, Christine ; Hatfull, Graham F. / Tales of diversity : Genomic and morphological characteristics of forty-six Arthrobacter phages. In: PLoS One. 2017 ; Vol. 12, No. 7.
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title = "Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages",
abstract = "The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45–68{\%}, compared to 63.4{\%} for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate.",
author = "Klyczek, {Karen K.} and Bonilla, {J. Alfred} and Deborah Jacobs-Sera and Adair, {Tamarah L.} and Patricia Afram and Allen, {Katherine G.} and Archambault, {Megan L.} and Aziz, {Rahat M.} and Bagnasco, {Filippa G.} and Ball, {Sarah L.} and Barrett, {Natalie A.} and Benjamin, {Robert C.} and Blasi, {Christopher J.} and Katherine Borst and Braun, {Mary A.} and Haley Broomell and Brown, {Conner B.} and Brynell, {Zachary S.} and Bue, {Ashley B.} and Burke, {Sydney O.} and William Casazza and Cautela, {Julia A.} and Kevin Chen and Chimalakonda, {Nitish S.} and Dylan Chudoff and Connor, {Jade A.} and Cross, {Trevor S.} and Curtis, {Kyra N.} and Dahlke, {Jessica A.} and Deaton, {Bethany M.} and Degroote, {Sarah J.} and Denigris, {Danielle M.} and Deruff, {Katherine C.} and Milan Dolan and David Dunbar and Egan, {Marisa S.} and Evans, {Daniel R.} and Fahnestock, {Abby K.} and Amal Farooq and Garrett Finn and Fratus, {Christopher R.} and Gaffney, {Bobby L.} and Garlena, {Rebecca A.} and Garrigan, {Kelly E.} and Gibbon, {Bryan C.} and Goedde, {Michael A.} and {Guerrero Bustamante}, {Carlos A.} and Melinda Harrison and Hartwell, {Megan C.} and Heckman, {Emily L.} and Jennifer Huang and Hughes, {Lee E.} and Hyduchak, {Kathryn M.} and Jacob, {Aswathi E.} and Machika Kaku and Karstens, {Allen W.} and Kenna, {Margaret A.} and Susheel Khetarpal and King, {Rodney A.} and Amanda Kobokovich and Hannah Kolev and Konde, {Sai A.} and Elizabeth Kriese and Lamey, {Morgan E.} and Lantz, {Carter N.} and Lapin, {Jonathan S.} and Lawson, {Temiloluwa O.} and Lee, {In Young} and Lee, {Scott M.} and Lee-Soety, {Julia Y.} and Lehmann, {Emily M.} and London, {Shawn C.} and Lopez, {A. Javier} and Lynch, {Kelly C.} and Mageeney, {Catherine M.} and Tetyana Martynyuk and Mathew, {Kevin J.} and Mavrich, {Travis N.} and McDaniel, {Christopher M.} and Hannah McDonald and McManus, {C. Joel} and Medrano, {Jessica E.} and Mele, {Francis E.} and Menninger, {Jennifer E.} and Miller, {Sierra N.} and Minick, {Josephine E.} and Nabua, {Courtney T.} and Napoli, {Caroline K.} and Martha Nkangabwa and Oates, {Elizabeth A.} and Ott, {Cassandra T.} and Pellerino, {Sarah K.} and Pinamont, {William J.} and Pirnie, {Ross T.} and Pizzorno, {Marie C.} and Plautz, {Emilee J.} and Pope, {Welkin H.} and Pruett, {Katelyn M.} and Gabbi Rickstrew and Rimple, {Patrick A.} and Rinehart, {Claire A.} and Robinson, {Kayla M.} and Rose, {Victoria A.} and Russell, {Daniel A.} and Schick, {Amelia M.} and Julia Schlossman and Schneider, {Victoria M.} and Sells, {Chloe A.} and Sieker, {Jeremy W.} and Silva, {Morgan P.} and Silvi, {Marissa M.} and Simon, {Stephanie E.} and Staples, {Amanda K.} and Steed, {Isabelle L.} and Stowe, {Emily L.} and Stueven, {Noah A.} and Swartz, {Porter T.} and Sweet, {Emma A.} and Sweetman, {Abigail T.} and Corrina Tender and Katrina Terry and Chrystal Thomas and Thomas, {Daniel S.} and Thompson, {Allison R.} and Lorianna Vanderveen and Rohan Varma and Vaught, {Hannah L.} and Vo, {Quynh D.} and Vonberg, {Zachary T.} and Ware, {Vassie C.} and Warrad, {Yasmene M.} and Wathen, {Kaitlyn E.} and Weinstein, {Jonathan L.} and Wyper, {Jacqueline F.} and Yankauskas, {Jakob R.} and Christine Zhang and Hatfull, {Graham F.}",
year = "2017",
month = "7",
day = "1",
doi = "10.1371/journal.pone.0180517",
language = "English (US)",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
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}

TY - JOUR

T1 - Tales of diversity

T2 - Genomic and morphological characteristics of forty-six Arthrobacter phages

AU - Klyczek, Karen K.

AU - Bonilla, J. Alfred

AU - Jacobs-Sera, Deborah

AU - Adair, Tamarah L.

AU - Afram, Patricia

AU - Allen, Katherine G.

AU - Archambault, Megan L.

AU - Aziz, Rahat M.

AU - Bagnasco, Filippa G.

AU - Ball, Sarah L.

AU - Barrett, Natalie A.

AU - Benjamin, Robert C.

AU - Blasi, Christopher J.

AU - Borst, Katherine

AU - Braun, Mary A.

AU - Broomell, Haley

AU - Brown, Conner B.

AU - Brynell, Zachary S.

AU - Bue, Ashley B.

AU - Burke, Sydney O.

AU - Casazza, William

AU - Cautela, Julia A.

AU - Chen, Kevin

AU - Chimalakonda, Nitish S.

AU - Chudoff, Dylan

AU - Connor, Jade A.

AU - Cross, Trevor S.

AU - Curtis, Kyra N.

AU - Dahlke, Jessica A.

AU - Deaton, Bethany M.

AU - Degroote, Sarah J.

AU - Denigris, Danielle M.

AU - Deruff, Katherine C.

AU - Dolan, Milan

AU - Dunbar, David

AU - Egan, Marisa S.

AU - Evans, Daniel R.

AU - Fahnestock, Abby K.

AU - Farooq, Amal

AU - Finn, Garrett

AU - Fratus, Christopher R.

AU - Gaffney, Bobby L.

AU - Garlena, Rebecca A.

AU - Garrigan, Kelly E.

AU - Gibbon, Bryan C.

AU - Goedde, Michael A.

AU - Guerrero Bustamante, Carlos A.

AU - Harrison, Melinda

AU - Hartwell, Megan C.

AU - Heckman, Emily L.

AU - Huang, Jennifer

AU - Hughes, Lee E.

AU - Hyduchak, Kathryn M.

AU - Jacob, Aswathi E.

AU - Kaku, Machika

AU - Karstens, Allen W.

AU - Kenna, Margaret A.

AU - Khetarpal, Susheel

AU - King, Rodney A.

AU - Kobokovich, Amanda

AU - Kolev, Hannah

AU - Konde, Sai A.

AU - Kriese, Elizabeth

AU - Lamey, Morgan E.

AU - Lantz, Carter N.

AU - Lapin, Jonathan S.

AU - Lawson, Temiloluwa O.

AU - Lee, In Young

AU - Lee, Scott M.

AU - Lee-Soety, Julia Y.

AU - Lehmann, Emily M.

AU - London, Shawn C.

AU - Lopez, A. Javier

AU - Lynch, Kelly C.

AU - Mageeney, Catherine M.

AU - Martynyuk, Tetyana

AU - Mathew, Kevin J.

AU - Mavrich, Travis N.

AU - McDaniel, Christopher M.

AU - McDonald, Hannah

AU - McManus, C. Joel

AU - Medrano, Jessica E.

AU - Mele, Francis E.

AU - Menninger, Jennifer E.

AU - Miller, Sierra N.

AU - Minick, Josephine E.

AU - Nabua, Courtney T.

AU - Napoli, Caroline K.

AU - Nkangabwa, Martha

AU - Oates, Elizabeth A.

AU - Ott, Cassandra T.

AU - Pellerino, Sarah K.

AU - Pinamont, William J.

AU - Pirnie, Ross T.

AU - Pizzorno, Marie C.

AU - Plautz, Emilee J.

AU - Pope, Welkin H.

AU - Pruett, Katelyn M.

AU - Rickstrew, Gabbi

AU - Rimple, Patrick A.

AU - Rinehart, Claire A.

AU - Robinson, Kayla M.

AU - Rose, Victoria A.

AU - Russell, Daniel A.

AU - Schick, Amelia M.

AU - Schlossman, Julia

AU - Schneider, Victoria M.

AU - Sells, Chloe A.

AU - Sieker, Jeremy W.

AU - Silva, Morgan P.

AU - Silvi, Marissa M.

AU - Simon, Stephanie E.

AU - Staples, Amanda K.

AU - Steed, Isabelle L.

AU - Stowe, Emily L.

AU - Stueven, Noah A.

AU - Swartz, Porter T.

AU - Sweet, Emma A.

AU - Sweetman, Abigail T.

AU - Tender, Corrina

AU - Terry, Katrina

AU - Thomas, Chrystal

AU - Thomas, Daniel S.

AU - Thompson, Allison R.

AU - Vanderveen, Lorianna

AU - Varma, Rohan

AU - Vaught, Hannah L.

AU - Vo, Quynh D.

AU - Vonberg, Zachary T.

AU - Ware, Vassie C.

AU - Warrad, Yasmene M.

AU - Wathen, Kaitlyn E.

AU - Weinstein, Jonathan L.

AU - Wyper, Jacqueline F.

AU - Yankauskas, Jakob R.

AU - Zhang, Christine

AU - Hatfull, Graham F.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45–68%, compared to 63.4% for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate.

AB - The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.S. infecting a single Arthrobacter sp. strain. These phages include representatives of all three virion morphologies, and Jasmine is the first sequenced podovirus of an actinobacterial host. The phages also span considerable sequence diversity, and can be grouped into 10 clusters according to their nucleotide diversity, and two singletons each with no close relatives. However, the clusters/singletons appear to be genomically well separated from each other, and relatively few genes are shared between clusters. Genome size varies from among the smallest of siphoviral phages (15,319 bp) to over 70 kbp, and G+C contents range from 45–68%, compared to 63.4% for the host genome. Although temperate phages are common among other actinobacterial hosts, these Arthrobacter phages are primarily lytic, and only the singleton Galaxy is likely temperate.

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