Tag-Mass: Specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag

R. Lemaire, J. Stauber, M. Wisztorski, C. Van Camp, A. Desmons, M. Deschamps, G. Proess, I. Rudlof, A. S. Woods, R. Day, M. Salzet, I. Fournier

Research output: Contribution to journalArticle

Abstract

MALDI tissue imaging of tissues has become a promising technique for tracking biomarkers while determining their location and structural characterization. We have now developed specific targeting probes (oligonucleotides, antibodies), named Tag-Mass. This approach is based on probes modified with a photocleavable linker coupled with a tag cleaved and detected using mass spectrometry. Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases.

Original languageEnglish (US)
Pages (from-to)2057-2067
Number of pages11
JournalJournal of Proteome Research
Volume6
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

Keywords

  • MALDI
  • Multiplex
  • Photocleavage
  • Proteome
  • Specific tissue imaging
  • Tag-Mass
  • Transcriptome

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

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  • Cite this

    Lemaire, R., Stauber, J., Wisztorski, M., Van Camp, C., Desmons, A., Deschamps, M., Proess, G., Rudlof, I., Woods, A. S., Day, R., Salzet, M., & Fournier, I. (2007). Tag-Mass: Specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag. Journal of Proteome Research, 6(6), 2057-2067. https://doi.org/10.1021/pr0700044