Taenia solium and Taenia ovis: Stage-specific expression of the vaccine antigen genes, TSOL18, TSOL16, and homologues, in oncospheres

Charles G. Gauci, Manuela R. Verástegui, Robert H. Gilman, Marshall W. Lightowlers

Research output: Contribution to journalArticlepeer-review

Abstract

Recombinant antigens that have been cloned from Taenia solium and Taenia ovis have been shown to be highly effective when used as vaccines against cysticercosis in the intermediate hosts. This study investigated the presence of mRNA encoding the TSOL18 and TSOL16 antigens in different life-cycle stages of T. solium, and their related homologues in T. ovis. Reverse transcription-PCR and Southern blotting demonstrated that the antigens are stage-specifically expressed in the oncosphere. The apparent absence of expression of TSOL18 in the metacestode life-cycle stage suggests that the vaccine based on this antigen targets exclusively the early stages in the development of the parasite.

Original languageEnglish (US)
Pages (from-to)272-275
Number of pages4
JournalExperimental Parasitology
Volume113
Issue number4
DOIs
StatePublished - Aug 2006

Keywords

  • Cestode
  • Cysticercosis
  • Echinococcus granulosus
  • Expression
  • Gene
  • Oncosphere
  • PCR
  • Parasite
  • RT-PCR
  • Taenia ovis
  • Taenia saginanta
  • Taenia solium
  • Vaccine
  • cDNA
  • complementary DNA
  • mRNA
  • messenger RNA
  • polymerase chain reaction
  • reverse transcription-PCR

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

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