T-lymphocytes expressing CC chemokine receptor-5 are increased in frail older adults

Umberto De Fanis, George Cho-Ching Wang, Neal S Fedarko, Jeremy D Walston, Vincenzo Casolaro, Sean Leng

Research output: Contribution to journalArticle

Abstract

OBJECTIVES: To evaluate the frequencies of T-lymphocytes expressing CC chemokine receptor-5 (CCR5+ T-cells) and their relationship with frailty in older adults. DESIGN: Case-control study with an age-, race-, and sex-matched design. SETTING: General Clinical Research Center. PARTICIPANTS: Community-dwelling adults aged 72 and older from Baltimore, Maryland. METHODS: Frailty was determined using five validated criteria: weakness, slow walking speed, fatigue, low physical activity, and weight loss. Those meeting three or more of these five criteria were defined as frail and those with none as nonfrail. Complete blood counts were performed to obtain peripheral lymphocyte counts using an automated (Coulter) counter. Peripheral blood was collected for surface immunofluorescent staining of CCR5 and other T-cell markers. RESULTS: Twenty-six frail and matched nonfrail participants (mean age±standard deviation 83.8±5.3, range 72-94) completed the study. Frail participants had higher CCR5+, CCR5+CD8+, and CCR5 +CD45RO- T-cell counts than matched nonfrail controls (349±160/mm3 vs 194±168/mm3, P=.02; 208±98/mm3 vs 105±62/mm3, P=.02; and 189±149/mm3 vs 52±36/mm3, P=.01; respectively). Furthermore, there was a trend toward graded increase in these T-cell counts across the frailty scores in frail participants (e.g., CCR5 +CD8+ counts of 123±52/mm3, 248±115/mm3, and 360±215/mm3 for those with frailty scores of 3, 4, and 5, respectively). CONCLUSION: These initial results suggest an expansion of the CCR5+ T-cell subpopulation in frailty. They provide a basis for further characterization of CCR5+ T-cells and their role in frailty, with potential therapeutic implications.

Original languageEnglish (US)
Pages (from-to)904-908
Number of pages5
JournalJournal of the American Geriatrics Society
Volume56
Issue number5
DOIs
StatePublished - May 2008

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CCR5 Receptors
Frail Elderly
T-Lymphocytes
Cell Count
Independent Living
Baltimore
Blood Cell Count
Lymphocyte Count
Fatigue
Case-Control Studies
Weight Loss
Exercise
Staining and Labeling

Keywords

  • CCR5 T-lymphocytes
  • Frailty
  • Inflammation

ASJC Scopus subject areas

  • Geriatrics and Gerontology

Cite this

T-lymphocytes expressing CC chemokine receptor-5 are increased in frail older adults. / De Fanis, Umberto; Wang, George Cho-Ching; Fedarko, Neal S; Walston, Jeremy D; Casolaro, Vincenzo; Leng, Sean.

In: Journal of the American Geriatrics Society, Vol. 56, No. 5, 05.2008, p. 904-908.

Research output: Contribution to journalArticle

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abstract = "OBJECTIVES: To evaluate the frequencies of T-lymphocytes expressing CC chemokine receptor-5 (CCR5+ T-cells) and their relationship with frailty in older adults. DESIGN: Case-control study with an age-, race-, and sex-matched design. SETTING: General Clinical Research Center. PARTICIPANTS: Community-dwelling adults aged 72 and older from Baltimore, Maryland. METHODS: Frailty was determined using five validated criteria: weakness, slow walking speed, fatigue, low physical activity, and weight loss. Those meeting three or more of these five criteria were defined as frail and those with none as nonfrail. Complete blood counts were performed to obtain peripheral lymphocyte counts using an automated (Coulter) counter. Peripheral blood was collected for surface immunofluorescent staining of CCR5 and other T-cell markers. RESULTS: Twenty-six frail and matched nonfrail participants (mean age±standard deviation 83.8±5.3, range 72-94) completed the study. Frail participants had higher CCR5+, CCR5+CD8+, and CCR5 +CD45RO- T-cell counts than matched nonfrail controls (349±160/mm3 vs 194±168/mm3, P=.02; 208±98/mm3 vs 105±62/mm3, P=.02; and 189±149/mm3 vs 52±36/mm3, P=.01; respectively). Furthermore, there was a trend toward graded increase in these T-cell counts across the frailty scores in frail participants (e.g., CCR5 +CD8+ counts of 123±52/mm3, 248±115/mm3, and 360±215/mm3 for those with frailty scores of 3, 4, and 5, respectively). CONCLUSION: These initial results suggest an expansion of the CCR5+ T-cell subpopulation in frailty. They provide a basis for further characterization of CCR5+ T-cells and their role in frailty, with potential therapeutic implications.",
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T1 - T-lymphocytes expressing CC chemokine receptor-5 are increased in frail older adults

AU - De Fanis, Umberto

AU - Wang, George Cho-Ching

AU - Fedarko, Neal S

AU - Walston, Jeremy D

AU - Casolaro, Vincenzo

AU - Leng, Sean

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N2 - OBJECTIVES: To evaluate the frequencies of T-lymphocytes expressing CC chemokine receptor-5 (CCR5+ T-cells) and their relationship with frailty in older adults. DESIGN: Case-control study with an age-, race-, and sex-matched design. SETTING: General Clinical Research Center. PARTICIPANTS: Community-dwelling adults aged 72 and older from Baltimore, Maryland. METHODS: Frailty was determined using five validated criteria: weakness, slow walking speed, fatigue, low physical activity, and weight loss. Those meeting three or more of these five criteria were defined as frail and those with none as nonfrail. Complete blood counts were performed to obtain peripheral lymphocyte counts using an automated (Coulter) counter. Peripheral blood was collected for surface immunofluorescent staining of CCR5 and other T-cell markers. RESULTS: Twenty-six frail and matched nonfrail participants (mean age±standard deviation 83.8±5.3, range 72-94) completed the study. Frail participants had higher CCR5+, CCR5+CD8+, and CCR5 +CD45RO- T-cell counts than matched nonfrail controls (349±160/mm3 vs 194±168/mm3, P=.02; 208±98/mm3 vs 105±62/mm3, P=.02; and 189±149/mm3 vs 52±36/mm3, P=.01; respectively). Furthermore, there was a trend toward graded increase in these T-cell counts across the frailty scores in frail participants (e.g., CCR5 +CD8+ counts of 123±52/mm3, 248±115/mm3, and 360±215/mm3 for those with frailty scores of 3, 4, and 5, respectively). CONCLUSION: These initial results suggest an expansion of the CCR5+ T-cell subpopulation in frailty. They provide a basis for further characterization of CCR5+ T-cells and their role in frailty, with potential therapeutic implications.

AB - OBJECTIVES: To evaluate the frequencies of T-lymphocytes expressing CC chemokine receptor-5 (CCR5+ T-cells) and their relationship with frailty in older adults. DESIGN: Case-control study with an age-, race-, and sex-matched design. SETTING: General Clinical Research Center. PARTICIPANTS: Community-dwelling adults aged 72 and older from Baltimore, Maryland. METHODS: Frailty was determined using five validated criteria: weakness, slow walking speed, fatigue, low physical activity, and weight loss. Those meeting three or more of these five criteria were defined as frail and those with none as nonfrail. Complete blood counts were performed to obtain peripheral lymphocyte counts using an automated (Coulter) counter. Peripheral blood was collected for surface immunofluorescent staining of CCR5 and other T-cell markers. RESULTS: Twenty-six frail and matched nonfrail participants (mean age±standard deviation 83.8±5.3, range 72-94) completed the study. Frail participants had higher CCR5+, CCR5+CD8+, and CCR5 +CD45RO- T-cell counts than matched nonfrail controls (349±160/mm3 vs 194±168/mm3, P=.02; 208±98/mm3 vs 105±62/mm3, P=.02; and 189±149/mm3 vs 52±36/mm3, P=.01; respectively). Furthermore, there was a trend toward graded increase in these T-cell counts across the frailty scores in frail participants (e.g., CCR5 +CD8+ counts of 123±52/mm3, 248±115/mm3, and 360±215/mm3 for those with frailty scores of 3, 4, and 5, respectively). CONCLUSION: These initial results suggest an expansion of the CCR5+ T-cell subpopulation in frailty. They provide a basis for further characterization of CCR5+ T-cells and their role in frailty, with potential therapeutic implications.

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