@article{6564fea6721b4f3eb61436da136a2220,
title = "T cells targeting carcinoembryonic antigen can mediate regression of metastatic colorectal cancer but induce severe transient colitis",
abstract = "Autologous T lymphocytes genetically engineered to express a murine T cell receptor (TCR) against human carcinoembryonic antigen (CEA) were administered to three patients with metastatic colorectal cancer refractory to standard treatments. All patients experienced profound decreases in serum CEA levels (74-99%), and one patient had an objective regression of cancer metastatic to the lung and liver. However, a severe transient inflammatory colitis that represented a dose limiting toxicity was induced in all three patients. This report represents the first example of objective regression of metastatic colorectal cancer mediated by adoptive T cell transfer and illustrates the successful use of a TCR, raised in human leukocyte antigen (HLA) transgenic mice, against a human tumor associated antigen. It also emphasizes the destructive power of small numbers of highly avid T cells and the limitations of using CEA as a target for cancer immunotherapy.",
author = "Parkhurst, {Maria R.} and Yang, {James C.} and Langan, {Russell C.} and Dudley, {Mark E.} and Nathan, {Debbie Ann N.} and Feldman, {Steven A.} and Davis, {Jeremy L.} and Morgan, {Richard A.} and Merino, {Maria J.} and Sherry, {Richard M.} and Hughes, {Marybeth S.} and Kammula, {Udai S.} and Phan, {Giao Q.} and Lim, {Ramona M.} and Wank, {Stephen A.} and Restifo, {Nicholas P.} and Robbins, {Paul F.} and Laurencot, {Carolyn M.} and Rosenberg, {Steven A.}",
note = "Funding Information: This work was funded through the NCI intramural program. The authors thank Arnold Mixon and Shawn Farid for performing FACS analyses; Linda Parker, Azam Nahvi, and Laura Deviller for expanding the CEA TCR transduced T cells in vitro for adoptive transfer; Hui Xu and Mary A. Black for producing GMP quality retrovirus encoding the CEA TCR; Yong Li for assisting with DNA analyses; Patricia Fetsch for immunohistochemical staining; Susan Schwarz and John Riley for performing ELISPOT assays; and all the clinical fellows and nursing staff in the Clinical Center of The National Institutes of Health who provided these patients with outstanding care.",
year = "2011",
month = mar,
doi = "10.1038/mt.2010.272",
language = "English (US)",
volume = "19",
pages = "620--626",
journal = "Molecular Therapy",
issn = "1525-0016",
publisher = "Nature Publishing Group",
number = "3",
}