TY - JOUR
T1 - T-cell subsets in the thyroids of mice developing autoimmune thyroiditis
AU - Creemers, Paula
AU - Giraldo, Alvaro A.
AU - Rose, Noel R.
AU - Kong, Yi Chi M.
N1 - Funding Information:
’ This study was supported by Grants AM 3 1827 and AM 30975 from the National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases,U .S. Department of Health and Human Services. 2 To whom correspondence should be addressed.
PY - 1984/9
Y1 - 1984/9
N2 - To examine the role of T-cell subsets in the development of thyroid lesions, female CBA/J mice were immunized with 60 μg mouse thyroglobulin (MTg) in 0.1 ml complete Freund's adjuvant in both hind footpads. The thyroids were removed 12-21 days later, pooled, and dispersed. The cell suspension was examined by membrane immunofluorescence for the distribution of Thy-1+, Lyt-1+, Lyt-2+, and sIg+ lymphocytes. For comparison, peripheral blood leukocytes (PBL) from the same animals were similarly examined. Throughout this 10-day interval, B cells in the thyroid were consistently below 5%, whereas B cells represented 19-24% of PBL. Thy-1+ cells in PBL ranged from 45 to 59%, whereas Thy-1+ cells in the thyroid were 37-50%. However, only thyroidal T cells showed a consistent decline with time and were replaced gradually by cells without T or B cell markers. In particular, there was a clear shift in the Lyt-1+:Lyt-2+ ratio from about 7 down to 2 in the thyroid as the early predominance of Lyt-1+ cells was followed by a relative increase in Lyt-2+ cells. Our results show that there is an accumulation of Lyt-1+ and Lyt-2+ cells in the infiltrated thyroid. These cells may include MTg-reactive, helper, and cytotoxic T cells which localize (or differentiate) in the thyroid and initiate the lesions.
AB - To examine the role of T-cell subsets in the development of thyroid lesions, female CBA/J mice were immunized with 60 μg mouse thyroglobulin (MTg) in 0.1 ml complete Freund's adjuvant in both hind footpads. The thyroids were removed 12-21 days later, pooled, and dispersed. The cell suspension was examined by membrane immunofluorescence for the distribution of Thy-1+, Lyt-1+, Lyt-2+, and sIg+ lymphocytes. For comparison, peripheral blood leukocytes (PBL) from the same animals were similarly examined. Throughout this 10-day interval, B cells in the thyroid were consistently below 5%, whereas B cells represented 19-24% of PBL. Thy-1+ cells in PBL ranged from 45 to 59%, whereas Thy-1+ cells in the thyroid were 37-50%. However, only thyroidal T cells showed a consistent decline with time and were replaced gradually by cells without T or B cell markers. In particular, there was a clear shift in the Lyt-1+:Lyt-2+ ratio from about 7 down to 2 in the thyroid as the early predominance of Lyt-1+ cells was followed by a relative increase in Lyt-2+ cells. Our results show that there is an accumulation of Lyt-1+ and Lyt-2+ cells in the infiltrated thyroid. These cells may include MTg-reactive, helper, and cytotoxic T cells which localize (or differentiate) in the thyroid and initiate the lesions.
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U2 - 10.1016/0008-8749(84)90037-6
DO - 10.1016/0008-8749(84)90037-6
M3 - Article
C2 - 6147201
AN - SCOPUS:0021233477
SN - 0008-8749
VL - 87
SP - 692
EP - 697
JO - Cellular Immunology
JF - Cellular Immunology
IS - 2
ER -