T-cell subsets in the thyroids of mice developing autoimmune thyroiditis

Paula Creemers, Alvaro A. Giraldo, Noel R. Rose, Yi Chi M Kong

Research output: Contribution to journalArticle

Abstract

To examine the role of T-cell subsets in the development of thyroid lesions, female CBA/J mice were immunized with 60 μg mouse thyroglobulin (MTg) in 0.1 ml complete Freund's adjuvant in both hind footpads. The thyroids were removed 12-21 days later, pooled, and dispersed. The cell suspension was examined by membrane immunofluorescence for the distribution of Thy-1+, Lyt-1+, Lyt-2+, and sIg+ lymphocytes. For comparison, peripheral blood leukocytes (PBL) from the same animals were similarly examined. Throughout this 10-day interval, B cells in the thyroid were consistently below 5%, whereas B cells represented 19-24% of PBL. Thy-1+ cells in PBL ranged from 45 to 59%, whereas Thy-1+ cells in the thyroid were 37-50%. However, only thyroidal T cells showed a consistent decline with time and were replaced gradually by cells without T or B cell markers. In particular, there was a clear shift in the Lyt-1+:Lyt-2+ ratio from about 7 down to 2 in the thyroid as the early predominance of Lyt-1+ cells was followed by a relative increase in Lyt-2+ cells. Our results show that there is an accumulation of Lyt-1+ and Lyt-2+ cells in the infiltrated thyroid. These cells may include MTg-reactive, helper, and cytotoxic T cells which localize (or differentiate) in the thyroid and initiate the lesions.

Original languageEnglish (US)
Pages (from-to)692-697
Number of pages6
JournalCellular Immunology
Volume87
Issue number2
DOIs
StatePublished - 1984

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Autoimmune Thyroiditis
T-Lymphocyte Subsets
Thyroid Gland
Leukocytes
B-Lymphocytes
Thyroglobulin
T-Lymphocytes
Inbred CBA Mouse
Freund's Adjuvant
Helper-Inducer T-Lymphocytes
Fluorescent Antibody Technique
Suspensions
Lymphocytes
Membranes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

T-cell subsets in the thyroids of mice developing autoimmune thyroiditis. / Creemers, Paula; Giraldo, Alvaro A.; Rose, Noel R.; Kong, Yi Chi M.

In: Cellular Immunology, Vol. 87, No. 2, 1984, p. 692-697.

Research output: Contribution to journalArticle

Creemers, Paula ; Giraldo, Alvaro A. ; Rose, Noel R. ; Kong, Yi Chi M. / T-cell subsets in the thyroids of mice developing autoimmune thyroiditis. In: Cellular Immunology. 1984 ; Vol. 87, No. 2. pp. 692-697.
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