T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis

Yujing Fu, Hongfei Lou, Chengshuo Wang, Wei Lou, Yang Wang, Tao Zheng, Luo Zhang

Research output: Contribution to journalArticle

Abstract

Background: This study aimed to investigate the influence of maternal allergy on cord blood regulatory and effector T cells and to evaluate their role as a predictor of atopic dermatitis (AD) during the first 2 yr of life. Methods: Seventy mother-infant pairs were recruited in this prospective birth cohort study (21 allergic and 49 non-allergic mothers). Cord blood samples were collected and assayed for the percentage of regulatory T cells (Treg), interferon-γ (IFN-γ), and interleukin-4 (IL-4) producing T cells (Th1 and Th2, respectively) using flow cytometry. Experiments were undertaken to assess the function of cord blood CD4+CD25+CD127- Treg cells by cell proliferation and cytokine responses. Their offspring at the age of 2 yr old were evaluated by dermatologists to determine whether they had AD. Results: During the first 2 yr of life, 15.7% of the children developed a physician-diagnosed AD. A significantly increased percentage of Th2 cell was observed in cord blood of newborns with maternal allergy. Treg/Th2 ratio significantly decreased among the offspring of allergic mothers. Treg cell-associated suppression of Th2 response was attenuated in Der p1-stimulated CD4+CD25- T cells from the offspring of allergic mothers. Children with reduced Th1/Th2 (p = 0.001, OR = 0.37) and Treg/Th2 (p = 0.001, OR = 0.47) ratio in cord blood had a higher risk of developing AD. Conclusion: Maternal allergic status is associated with increased percentage of IL-4+CD4+ T cells and a reduced Treg/Th2 ratio in cord blood at their children's birth, which may predispose to an increased risk for developing AD.

Original languageEnglish (US)
Pages (from-to)178-186
Number of pages9
JournalPediatric Allergy and Immunology
Volume24
Issue number2
DOIs
StatePublished - Mar 2013

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T-Lymphocyte Subsets
Atopic Dermatitis
Fetal Blood
Hypersensitivity
Mothers
Regulatory T-Lymphocytes
T-Lymphocytes
Interleukin-4
Parturition
Th2 Cells
Interferons
Flow Cytometry
Cohort Studies
Cell Proliferation
Newborn Infant
Cytokines
Physicians

Keywords

  • Atopic dermatitis
  • Cord blood
  • Interferon-γ positive cells
  • Interleukin-4 positive cells
  • Regulatory T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pediatrics, Perinatology, and Child Health
  • Immunology

Cite this

T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis. / Fu, Yujing; Lou, Hongfei; Wang, Chengshuo; Lou, Wei; Wang, Yang; Zheng, Tao; Zhang, Luo.

In: Pediatric Allergy and Immunology, Vol. 24, No. 2, 03.2013, p. 178-186.

Research output: Contribution to journalArticle

Fu, Yujing ; Lou, Hongfei ; Wang, Chengshuo ; Lou, Wei ; Wang, Yang ; Zheng, Tao ; Zhang, Luo. / T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis. In: Pediatric Allergy and Immunology. 2013 ; Vol. 24, No. 2. pp. 178-186.
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AU - Fu, Yujing

AU - Lou, Hongfei

AU - Wang, Chengshuo

AU - Lou, Wei

AU - Wang, Yang

AU - Zheng, Tao

AU - Zhang, Luo

PY - 2013/3

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N2 - Background: This study aimed to investigate the influence of maternal allergy on cord blood regulatory and effector T cells and to evaluate their role as a predictor of atopic dermatitis (AD) during the first 2 yr of life. Methods: Seventy mother-infant pairs were recruited in this prospective birth cohort study (21 allergic and 49 non-allergic mothers). Cord blood samples were collected and assayed for the percentage of regulatory T cells (Treg), interferon-γ (IFN-γ), and interleukin-4 (IL-4) producing T cells (Th1 and Th2, respectively) using flow cytometry. Experiments were undertaken to assess the function of cord blood CD4+CD25+CD127- Treg cells by cell proliferation and cytokine responses. Their offspring at the age of 2 yr old were evaluated by dermatologists to determine whether they had AD. Results: During the first 2 yr of life, 15.7% of the children developed a physician-diagnosed AD. A significantly increased percentage of Th2 cell was observed in cord blood of newborns with maternal allergy. Treg/Th2 ratio significantly decreased among the offspring of allergic mothers. Treg cell-associated suppression of Th2 response was attenuated in Der p1-stimulated CD4+CD25- T cells from the offspring of allergic mothers. Children with reduced Th1/Th2 (p = 0.001, OR = 0.37) and Treg/Th2 (p = 0.001, OR = 0.47) ratio in cord blood had a higher risk of developing AD. Conclusion: Maternal allergic status is associated with increased percentage of IL-4+CD4+ T cells and a reduced Treg/Th2 ratio in cord blood at their children's birth, which may predispose to an increased risk for developing AD.

AB - Background: This study aimed to investigate the influence of maternal allergy on cord blood regulatory and effector T cells and to evaluate their role as a predictor of atopic dermatitis (AD) during the first 2 yr of life. Methods: Seventy mother-infant pairs were recruited in this prospective birth cohort study (21 allergic and 49 non-allergic mothers). Cord blood samples were collected and assayed for the percentage of regulatory T cells (Treg), interferon-γ (IFN-γ), and interleukin-4 (IL-4) producing T cells (Th1 and Th2, respectively) using flow cytometry. Experiments were undertaken to assess the function of cord blood CD4+CD25+CD127- Treg cells by cell proliferation and cytokine responses. Their offspring at the age of 2 yr old were evaluated by dermatologists to determine whether they had AD. Results: During the first 2 yr of life, 15.7% of the children developed a physician-diagnosed AD. A significantly increased percentage of Th2 cell was observed in cord blood of newborns with maternal allergy. Treg/Th2 ratio significantly decreased among the offspring of allergic mothers. Treg cell-associated suppression of Th2 response was attenuated in Der p1-stimulated CD4+CD25- T cells from the offspring of allergic mothers. Children with reduced Th1/Th2 (p = 0.001, OR = 0.37) and Treg/Th2 (p = 0.001, OR = 0.47) ratio in cord blood had a higher risk of developing AD. Conclusion: Maternal allergic status is associated with increased percentage of IL-4+CD4+ T cells and a reduced Treg/Th2 ratio in cord blood at their children's birth, which may predispose to an increased risk for developing AD.

KW - Atopic dermatitis

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KW - Interferon-γ positive cells

KW - Interleukin-4 positive cells

KW - Regulatory T cells

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