T-cell response to myelin basic protein and lipid-bound myelin basic protein in patients with multiple sclerosis and healthy donors

Benedetta Mazzanti, Marco Vergelli, Paolo Riccio, Roland Martin, Henry F. McFarland, Grazia M. Liuzzi, Luigi Amaducci, Luca Massacesi

Research output: Contribution to journalArticlepeer-review

Abstract

An autoimmune T-cell response to myelin proteins is thought to be involved in the pathogenesis of multiple sclerosis (MS) and myelin basic protein (MBP) is the most widely studied potential target antigen. We investigated the T-cell response to MBP in MS patients and controls using two different molecular forms of the protein: the classical hydrophilic MBP (lipid-free MBP, LF-MBP) and a lipid-bound, native-like preparation of MBP isolated in a molecular form retaining the binding to all myelin lipids (lipid-bound-MBP, LB-MBP). Short term T-cell lines (TCL) were generated using either LF- or LB-MBP and tested for their reactivity to the in vitro stimulating antigen. No differences were detected between MS patients and healthy donors in the percentage of T-cell cultures responsive to the LF- MBP. In contrast, the number of LB-MBP reactive cultures was higher in MS patients than in controls. This difference was almost entirely due to the presence of high numbers of LB-MBP-specific TCL in MS patients which did not cross-react with LF-MBP and were not present in healthy subjects. LB-MBP may represent a novel antigen worth to be investigated in MS.

Original languageEnglish (US)
Pages (from-to)96-100
Number of pages5
JournalJournal of Neuroimmunology
Volume82
Issue number1
DOIs
StatePublished - Feb 1998
Externally publishedYes

Keywords

  • Lipids
  • Multiple sclerosis
  • Myelin basic protein
  • T-cells

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Fingerprint Dive into the research topics of 'T-cell response to myelin basic protein and lipid-bound myelin basic protein in patients with multiple sclerosis and healthy donors'. Together they form a unique fingerprint.

Cite this