T cell repertoire in asthmatic airways before and after ragweed challenge

V. V. Yurovskv, E. J M Weersink, D. S. Postma, E. R. Bleecker, B. White

Research output: Contribution to journalArticle

Abstract

T cell repertoire in the blood and bronchoalveolar lavage (BAL) fluids from six atopic asthmatics was tested before and after segmentai ragweed challenge. Analyses of cell numbers, differential, and T cell subsets in BAL fluids showed that ragweed challenge was associated with preferential homing of CD4+ T cells to the lungs. A reverse transcriptase-PCR was used to amplify TCR V gene transcripts from unfractionated, CD4+, and CD8+ T cells. Levels of expression of 22 Va and 25 Vβ gene families in BAL fluids were similar to those of the same V genes in blood. Ragweed challenge did not change the levels of expression of these V gene families. The clonality of T cells was estimated by analyzing the diversity of TCR V(D)-J junctional region lengths associated with each Va and Vβ gene family, using sequencing gel electrophoresis. Most V gene families in blood and BAL fluids were associated with multiple junctional region lengths before and after ragweed challenge, indicating polyclonal expression. However, some V gene families were expressed in an oligoclonal manner in unfractionated, CD4+, and CD8+ T cells in BAL fluids before ragweed challenge, as indicated by a few predominant junctional region lengths. Most of these V genes families became polyclonal after challenge. Some V gene families became oligoclonal or developed a new oligoclonal pattern of junctional region lengths in BAL T cells after ragweed challenge. This occurred in both CD4+ and CD8+ T cells and was confirmed by DNA sequence analysis. These findings show that ragweed challenge is associated with polyclonal influx and oligoclonal activation of both CD4+ and CD8+ T cells in the lungs.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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    Yurovskv, V. V., Weersink, E. J. M., Postma, D. S., Bleecker, E. R., & White, B. (1996). T cell repertoire in asthmatic airways before and after ragweed challenge. FASEB Journal, 10(3).