T cell infiltration and MHC I and II expression in the presence of tumor antigens: An immunohistochemical study in patients with serous epithelial ovarian cancer

Hans W. Nijman, Paul J. Van Diest, Ria J J Poort-Keesom, Silvia Von Mensdorff-Pouilly, Rob A. Verstraeten, Alain Kummer, Chris J L M Meijer, Cornelis J M Melief, Jo Hilgers, Peter Kenemans

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Ovarian cancer is a frequent cause of death among women with gynaecologic malignancies despite the introduction of combination chemotherapy. There is therefore a need for new therapeutic strategies for patients with ovarian cancer, such as cellular immunotherapy. In this immunohistochemical study we analysed the expression of three tumor antigens, p53, HER-2/neu and MUC-1 in relation to the expression of major histocompatibility complex (MHC) class I and II on tumor cells, and we searched for the presence of (activated) immune effector cells at the tumor site. Study design: The study was carried out retrospectively in tumor tissue from 29 patients with serous ovarian cancer. Material used had been formalin fixed and paraffin embedded. Material had been obtained from 15 patients at staging laparotomy and from 14 patients during second look or intervention laparotomy. Results: A positive staining for p53 was found in 19/29 (66%) of the tumors, with a high positivity in 13/29 (45%). HER-2/neu and MUC-1 staining was positive in 8/29 (28%) and 21/28 (75%), respectively. Downregulation of MHC class I on tumor cells was found in a minority of the patients, beta-2-microglobin (β2m) was expressed on tumor cells in all patients. High staining for CD45RO correlated with a high positive staining for granzyme-B (R=0.40, P=0.04) and TIA-1 (R=0.39, P=0.04). A statistically significant better survival in the group with lower stage of disease was found. Conclusions: As only three out of 29 patients were negative for the tumor antigens p53, HER-2/neu and MUC-1, immunotherapy aiming at all three could serve almost all patients with ovarian cancer. We found that granzyme-B, TIA-1 and CD45RO+ T cells are present in the tumor biopsies, increasing this number by immunotherapy may be beneficial. The immune escape mechanism by MHC class I and β2m downregulation seems to be of minor importance. Our data support the view that immunotherapy may offer new possibilities with high specificity in ovarian cancer.

Original languageEnglish (US)
Pages (from-to)114-120
Number of pages7
JournalEuropean Journal of Obstetrics Gynecology and Reproductive Biology
Volume94
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Human
  • Immunology
  • Ovarian cancer
  • Tumor antigens

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

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