T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis

Thomas G. Sharp, David H. Sachs, Anthony S. Fauci, Gerald L. Messerschmidt, Steven A. Rosenberg

Research output: Contribution to journalArticle

Abstract

Human bone marrow was harvested from surgically resected bones of 25 patients and was tested for the presence of mature T cells. An average of 6.5% (±1.2% SE) of nucleated bone marrow cells formed spontaneous rosettes with sheep red blood cells. Functional T cells in bone marrow were also identified by characteristic responses to alloantigens and the T cell mitogens concanavalin A (Con A) and phytohemagglutinin (PHA). The ability of three monoclonal antibodies (OKT.3, Lyt-3, and (Leu-1) to lyse peripheral T cells in the presence of rabbit complement was examined. All three reagents were found to be specifically lytic for mature T cells in peripheral blood. One reagent (Leu-1) was selected for use in depletion of T cells in human bone marrow. Seven of 10 experiments performed showed sufficient T cell responses to be evaluable. In all of these experiments, a marked reduction of T cells and T cell functions was observed. On the average, E rosettes were reduced 89.2% (±3.0% SE) below medium controls while the mean PHA, Con A, and mixed lymphocyte culture (MLC) activity were completely eliminated to levels below background. In four experiments, colony-forming units (CFU-GM) in bone marrow were assayed following treatment with Leu-1 and showed a mean increase of 194% (±32% SE) over medium controls. Since mature T cells are thought to be responsible for graft-versus-host disease in allogeneic bone marrow transplantation, this method of T cell depletion may be useful for preparing marrow for human bone marrow transplants.

Original languageEnglish (US)
Pages (from-to)112-120
Number of pages9
JournalTransplantation
Volume35
Issue number2
StatePublished - 1983
Externally publishedYes

Fingerprint

Bone Marrow
Monoclonal Antibodies
T-Lymphocytes
Phytohemagglutinins
Concanavalin A
Granulocyte-Macrophage Progenitor Cells
Isoantigens
Homologous Transplantation
Graft vs Host Disease
Bone Marrow Transplantation
Mitogens
Bone Marrow Cells
Sheep
Stem Cells
Erythrocytes
Lymphocytes
Rabbits
Transplants
Bone and Bones

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Sharp, T. G., Sachs, D. H., Fauci, A. S., Messerschmidt, G. L., & Rosenberg, S. A. (1983). T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis. Transplantation, 35(2), 112-120.

T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis. / Sharp, Thomas G.; Sachs, David H.; Fauci, Anthony S.; Messerschmidt, Gerald L.; Rosenberg, Steven A.

In: Transplantation, Vol. 35, No. 2, 1983, p. 112-120.

Research output: Contribution to journalArticle

Sharp, TG, Sachs, DH, Fauci, AS, Messerschmidt, GL & Rosenberg, SA 1983, 'T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis', Transplantation, vol. 35, no. 2, pp. 112-120.
Sharp TG, Sachs DH, Fauci AS, Messerschmidt GL, Rosenberg SA. T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis. Transplantation. 1983;35(2):112-120.
Sharp, Thomas G. ; Sachs, David H. ; Fauci, Anthony S. ; Messerschmidt, Gerald L. ; Rosenberg, Steven A. / T cell depletion of human bone marrow using monoclonal antibody and complement-mediated lysis. In: Transplantation. 1983 ; Vol. 35, No. 2. pp. 112-120.
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