T-cell activation is correlated with monocyte activation in HCV/HIV coinfection and declines during HCV direct-acting antiviral therapy

Ann W.N. Auma, Carey Shive, Sofi Damjanovska, Corinne Kowal, Daniel E. Cohen, Debika Bhattacharya, Beverly Alston-Smith, Melissa Osborne, Robert Kalayjian, Ashwin Balagopal, Mark Sulkowski, David Wyles, Donald D. Anthony

Research output: Contribution to journalArticlepeer-review


Background: Immune activation markers associate with morbidity and mortality in HIV and hepatitis C virus (HCV) infection. We investigated how T-cell and monocyte activation are related over the course of HCV direct-acting antiviral (DAA) therapy during HCV/HIV coinfection. Methods: Peripheral blood mononuclear cells from AIDS Clinical Trials Group (ACTG) A5329 participants and a single-site separate cohort treated with DAAs were analyzed for central memory (CM)/effector memory (EM) T-cell subsets, monocyte subsets, and cell activation (CD38 and HLA-DR expression) before, during, and after therapy. Results: Before therapy, classical and inflammatory monocyte subset HLA-DR expression positively correlated with absolute counts and frequencies of CD38+HLA-DR+-expressing CD4+ and CD8 T cells and corresponding CM and EM subsets. After therapy initiation, CD38+HLA-DR+ co-expression on CD4+ and CD8+ memory T cells decreased by 12 weeks and 36 weeks, and plasma sCD14 positively correlated with CD38+HLA-DR+ CD4+ and CD4+CM T-cell frequencies. Monocyte subset activation remained similar over time. Conclusions: During HCV/HIV coinfection, memory T-cell activation is associated with monocyte subset activation, consistent with related underlying mechanisms. Following therapy initiation, memory T-cell, but not monocyte, activation decreased. Residual CD4+ T-cell activation after therapy completion is associated with sCD14, potentially linking the remaining CD4+ T-cell activation to residual factors driving activation in antiretroviral therapy-controlled HIV.

Original languageEnglish (US)
JournalOpen Forum Infectious Diseases
Issue number4
StatePublished - Apr 1 2021


  • DAA therapy
  • T cell
  • hepatitis C and HIV coinfection
  • human
  • immunity
  • inflammation
  • monocyte

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology


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