T. Brucei RNA editing: Adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage

Jorge Cruz-Reyes, Laura N. Rusché, Kenneth J. Piller, Barbara Sollner-Webb

Research output: Contribution to journalArticlepeer-review

Abstract

In the currently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insert ion and U-deletion cycles begin with a common kind of gRNA-directed cleavage. However, natural, altered, and mutationally interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated by ATP and ADP, while U-insertional cleavage shows completely reverse nucleotide effects. The adenosine nucleotides' effects appear to be allosteric and determined solely by sequences immediately adjacent to the anchor duplex. Both U-deletional and U-insertional cleavages are reasonably active at physiological mitochondrial ATP concentration. Notably, ATP and ADP markedly stimulate complete U-deletion and inhibit U-insertion reactions, reflecting their effects on cleavage. These plus previous results suggest that U deletion and U insertion are remarkably distinct.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalMolecular cell
Volume1
Issue number3
DOIs
StatePublished - Feb 1998

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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