TY - JOUR
T1 - T. Brucei RNA editing
T2 - Adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage
AU - Cruz-Reyes, Jorge
AU - Rusché, Laura N.
AU - Piller, Kenneth J.
AU - Sollner-Webb, Barbara
N1 - Funding Information:
We thank Drs. Paul Englund, Scott Seiwert, Ken Stuart, and members of the Sollner-Webb lab for helpful discussions. We also thank Drs. Noreen Williams and C. C. Wang for providing unpublished information about trypanosome mitochondrial ATP concentration. Alevtina Zhelonkina provided expert technical assistance.This work was supported by NIH grant GM34231. J. C. R. was supported by a PEW Latin-American Fellowship Grant P0052SC, and L. N. R. by a Howard Hughes Predoctoral Fellowship.
PY - 1998/2
Y1 - 1998/2
N2 - In the currently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insert ion and U-deletion cycles begin with a common kind of gRNA-directed cleavage. However, natural, altered, and mutationally interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated by ATP and ADP, while U-insertional cleavage shows completely reverse nucleotide effects. The adenosine nucleotides' effects appear to be allosteric and determined solely by sequences immediately adjacent to the anchor duplex. Both U-deletional and U-insertional cleavages are reasonably active at physiological mitochondrial ATP concentration. Notably, ATP and ADP markedly stimulate complete U-deletion and inhibit U-insertion reactions, reflecting their effects on cleavage. These plus previous results suggest that U deletion and U insertion are remarkably distinct.
AB - In the currently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insert ion and U-deletion cycles begin with a common kind of gRNA-directed cleavage. However, natural, altered, and mutationally interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated by ATP and ADP, while U-insertional cleavage shows completely reverse nucleotide effects. The adenosine nucleotides' effects appear to be allosteric and determined solely by sequences immediately adjacent to the anchor duplex. Both U-deletional and U-insertional cleavages are reasonably active at physiological mitochondrial ATP concentration. Notably, ATP and ADP markedly stimulate complete U-deletion and inhibit U-insertion reactions, reflecting their effects on cleavage. These plus previous results suggest that U deletion and U insertion are remarkably distinct.
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U2 - 10.1016/S1097-2765(00)80040-4
DO - 10.1016/S1097-2765(00)80040-4
M3 - Article
C2 - 9660924
AN - SCOPUS:0031993321
SN - 1097-2765
VL - 1
SP - 401
EP - 409
JO - Molecular cell
JF - Molecular cell
IS - 3
ER -