Systemic treatment of focal brain injury in the rat by human umbilical cord blood cells being at different level of neural commitment

Elzbieta Gornicka-Pawlak, Miroslaw Janowski, Aleksandra Habich, Anna Jablonska, Katarzyna Drela, Hanna Kozlowska, Barbara Lukomska, Joanna Sypecka, Krystyna Domanska-Janik

Research output: Contribution to journalArticle

Abstract

The aim of the study was to evaluate therapeutic effectiveness of intra-arterial infusion of human umbilical cord blood (HUCB) derived cells at different stages of their neural conversion. Freshly isolated mononuclear cells (D-0), neurally directed progenitors (D-3) and neural-like stem cells derived from umbilical cord blood (NSC) were compared. Focal brain damage was induced in rats by stereotactic injection of ouabain into dorsolateral striatum Three days later 107 of different subsets of HUCB cells were infused into the right internal carotid artery. Following surgery rats were housed in enriched environment for 30 days. Behavioral assessment consisted of tests for sensorimotor deficits (walking beam, rotarod, vibrissae elicited forelimb placing, apomorphine induced rotations), cognitive impairments (habit learning and object recognition) and exploratory behavior (open field). Thirty days after surgery the lesion volume was measured and the presence of donor cells was detected in the brain at mRNA level. At the same time immunohistochemical analysis of brain tissue was performed to estimate the local tissue response of ouabain injured rats and its modulation after HUCB cells systemic treatment. Functional effects of different subsets of cord blood cells shared substantial diversity in various behavioral tests. An additional analysis showed that D-0 HUCB cells were the most effective in functional restoration and reduction of brain lesion volume. None of transplanted cord blood derived cell fractions were detected in rat's brains at 30th day after treatment. This may suggest that the mechanism(s) underlying positive effects of HUCB derived cell may concern the other than direct neural cell supplementation. In addition increased immunoreactivity of markers indicating local cells proliferation and migration suggests stimulation of endogenous reparative processes by HUCB D-0 cell interarterial infusion.

Original languageEnglish (US)
Pages (from-to)46-64
Number of pages19
JournalActa Neurobiologiae Experimentalis
Volume71
Issue number1
StatePublished - May 2 2011

Keywords

  • Cord blood cells
  • Focal brain injury
  • Functional recovery
  • Neurogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

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    Gornicka-Pawlak, E., Janowski, M., Habich, A., Jablonska, A., Drela, K., Kozlowska, H., Lukomska, B., Sypecka, J., & Domanska-Janik, K. (2011). Systemic treatment of focal brain injury in the rat by human umbilical cord blood cells being at different level of neural commitment. Acta Neurobiologiae Experimentalis, 71(1), 46-64.