Systematic genome instability screens in yeast and their potential relevance to cancer

Karen W.Y. Yuen, Cheryl D. Warren, Ou Chen, Teresa Kwok, Phil Hieter, Forrest A. Spencer

Research output: Contribution to journalArticle

Abstract

To systematically identify genes that maintain genome structure, yeast knockout mutants were examined by using three assays that followed marker inheritance in different chromosomal contexts. These screens identified 130 null mutant strains exhibiting chromosome instability (CIN) phenotypes. Differences in both phenotype severity and assay specificity were observed. The results demonstrate the advantages of using complementary assays to comprehensively identify genome maintenance determinants. Genome structure was important in determining the spectrum of gene and pathway mutations causing a chromosome instability phenotype. Protein similarity identified homologues in other species, including human genes with relevance to cancer. This extensive genome instability catalog can be combined with emerging genetic interaction data from yeast to support the identification of candidate targets for therapeutic elimination of chromosomally unstable cancer cells by selective cell killing.

Original languageEnglish (US)
Pages (from-to)3925-3930
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number10
DOIs
StatePublished - Mar 6 2007

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Systematic genome instability screens in yeast and their potential relevance to cancer'. Together they form a unique fingerprint.

  • Cite this