Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression

Joanne Topol, Charles R. Dearolf, Kulkarni Prakash, Carl S. Parker

Research output: Contribution to journalArticle

Abstract

A complex array of activator and represser elements located within 669 bp proximal to the fushi tarazu (ftz) transcriptional start site is sufficient to generate the "zebra-stripe" expression pattern characteristic of the ftz gene. P-element-mediated transformation and ftz promoter/lacZ fusion genes were used to characterize, in detail, several of these transcriptional control elements. By reconstructing promoters with synthetic oligonucleotides containing cis-regulators of stripe expression, we show that these regulatory sites can function as independent units to direct position-specific transcription in the Drosophila embryo. In particular, we demonstrate that multiple copies of a positive regulatory site can mediate expression in both the odd- and even-numbered parasegments throughout most of the germ band and that negative regulatory sites can transform a continuous pattern of gene expression into discrete stripes. The reconstructed promoter system presented provides an effective means of studying molecular mechanisms governing spatially restricted transcription in the early embryo.

Original languageEnglish (US)
Pages (from-to)855-867
Number of pages13
JournalGenes & development
Volume5
Issue number5
StatePublished - 1991
Externally publishedYes

Fingerprint

Equidae
Oligonucleotides
Drosophila
Embryonic Structures
Lac Operon
Gene Expression
Genes

Keywords

  • Drosophila
  • Ftz gene
  • Repression
  • Stripe expression

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

Topol, J., Dearolf, C. R., Prakash, K., & Parker, C. S. (1991). Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression. Genes & development, 5(5), 855-867.

Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression. / Topol, Joanne; Dearolf, Charles R.; Prakash, Kulkarni; Parker, Carl S.

In: Genes & development, Vol. 5, No. 5, 1991, p. 855-867.

Research output: Contribution to journalArticle

Topol, J, Dearolf, CR, Prakash, K & Parker, CS 1991, 'Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression', Genes & development, vol. 5, no. 5, pp. 855-867.
Topol, Joanne ; Dearolf, Charles R. ; Prakash, Kulkarni ; Parker, Carl S. / Synthetic oligonucleotides recreate Drosophila fushi tarazu zebra-stripe expression. In: Genes & development. 1991 ; Vol. 5, No. 5. pp. 855-867.
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