Synthetic Nanofiber-Reinforced Amniotic Membrane via Interfacial Bonding

Huanhuan Liu, Zhengbing Zhou, Hui Lin, Juan Wu, Brian Ginn, Ji Suk Choi, Xuesong Jiang, Liam Chung, Jennifer Hartt Elisseeff, Samuel Chi-Hung Yiu, Hai Quan Mao

Research output: Contribution to journalArticle

Abstract

Severe damage to the ocular surface can result in limbal stem cell (LSC) deficiency, which contributes to loss of corneal clarity, potential vision loss, chronic pain, photophobia, and keratoplasty failure. Human amniotic membrane (AM) is the most effective substrate for LSC transplantation to treat patients with LSC deficiency. However, the widespread use of the AM in the clinic remains a challenge because of the high cost for preserving freshly prepared AM and the weak mechanical strength of lyophilized AM. Here, we developed a novel composite membrane consisting of an electrospun bioabsorbable polymer fiber mesh bonded to a decellularized AM (dAM) sheet through interfacial conjugation. This membrane engineering approach drastically improved the tensile property and toughness of dAM, preserved similar levels of bioactivities as the dAM itself in supporting LSC attachment, growth, and maintenance, and retained significant anti-inflammatory capacity. These results demonstrate that the lyophilized nanofiber-dAM composite membrane offers superior mechanical properties for easy handling and suturing to the dAM, while presenting biochemical cues and basement membrane structure to facilitate LSC transplantation. This composite membrane exhibits major advantages for clinical applications in treating soft tissue damage and LSC deficiency.

Original languageEnglish (US)
Pages (from-to)14559-14569
Number of pages11
JournalACS Applied Materials and Interfaces
Volume10
Issue number17
DOIs
StatePublished - May 2 2018

Fingerprint

Nanofibers
Stem cells
Membranes
Composite membranes
Membrane structures
Bioactivity
Tensile properties
Toughness
Strength of materials
Polymers
Anti-Inflammatory Agents
Tissue
Mechanical properties
Fibers
Substrates
Costs

Keywords

  • amniotic membrane
  • composite membrane
  • electrospinning
  • limbal stem cell transplantation
  • macrophage phenotype
  • nanofibers

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Synthetic Nanofiber-Reinforced Amniotic Membrane via Interfacial Bonding. / Liu, Huanhuan; Zhou, Zhengbing; Lin, Hui; Wu, Juan; Ginn, Brian; Choi, Ji Suk; Jiang, Xuesong; Chung, Liam; Elisseeff, Jennifer Hartt; Yiu, Samuel Chi-Hung; Mao, Hai Quan.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 17, 02.05.2018, p. 14559-14569.

Research output: Contribution to journalArticle

Liu, Huanhuan ; Zhou, Zhengbing ; Lin, Hui ; Wu, Juan ; Ginn, Brian ; Choi, Ji Suk ; Jiang, Xuesong ; Chung, Liam ; Elisseeff, Jennifer Hartt ; Yiu, Samuel Chi-Hung ; Mao, Hai Quan. / Synthetic Nanofiber-Reinforced Amniotic Membrane via Interfacial Bonding. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 17. pp. 14559-14569.
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