Synthesis, radiometal labeling and in vitro evaluation of a targeted PPIX derivative

Babak Behnam Azad; Choi Fong Cho; John D. Lewis; Leonard G. Luyt

doi:10.1016/j.apradiso.2011.11.054

Synthesis, radiometal labeling and in vitro evaluation of a targeted PPIX derivative

Babak Behnam Azad, Choi Fong Cho, John D. Lewis, Leonard G. Luyt

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

In pursuit of a single molecule with potential applications for both in vitro fluorescence microscopy and in vivo PET imaging, novel targeted ^69/71Ga/ ⁶⁸Ga-protoporphyrin IX (PPIX) probes were developed. Optical analysis, fluorescence microscopy and radiolabeling with gallium-68 were performed to confirm potential applications. The use of a targeted-PPIX ring, in conjunction with ^69/71Ga or ⁶⁸Ga, eliminates the need for the attachment of multiple imaging tags, allowing for either in vitro fluorescent-based evaluation or in vivo nuclear-based imaging.

Original language	English (US)
Pages (from-to)	505-511
Number of pages	7
Journal	Applied Radiation and Isotopes
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/j.apradiso.2011.11.054
State	Published - Mar 2012
Externally published	Yes

Keywords

Fluorescence microscopy
Gallium-68
Metal conjugates
Molecular imaging
PPIX
Radiolabeling

ASJC Scopus subject areas

Radiation

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10.1016/j.apradiso.2011.11.054

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title = "Synthesis, radiometal labeling and in vitro evaluation of a targeted PPIX derivative",

abstract = "In pursuit of a single molecule with potential applications for both in vitro fluorescence microscopy and in vivo PET imaging, novel targeted 69/71Ga/ 68Ga-protoporphyrin IX (PPIX) probes were developed. Optical analysis, fluorescence microscopy and radiolabeling with gallium-68 were performed to confirm potential applications. The use of a targeted-PPIX ring, in conjunction with 69/71Ga or 68Ga, eliminates the need for the attachment of multiple imaging tags, allowing for either in vitro fluorescent-based evaluation or in vivo nuclear-based imaging.",

keywords = "Fluorescence microscopy, Gallium-68, Metal conjugates, Molecular imaging, PPIX, Radiolabeling",

author = "{Behnam Azad}, Babak and Cho, {Choi Fong} and Lewis, {John D.} and Luyt, {Leonard G.}",

note = "Funding Information: The authors gratefully acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) . ",

year = "2012",

month = mar,

doi = "10.1016/j.apradiso.2011.11.054",

language = "English (US)",

volume = "70",

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journal = "Applied Radiation and Isotopes",

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T1 - Synthesis, radiometal labeling and in vitro evaluation of a targeted PPIX derivative

AU - Behnam Azad, Babak

AU - Cho, Choi Fong

AU - Lewis, John D.

AU - Luyt, Leonard G.

N1 - Funding Information: The authors gratefully acknowledge funding from the Natural Sciences and Engineering Research Council of Canada (NSERC) .

PY - 2012/3

Y1 - 2012/3

N2 - In pursuit of a single molecule with potential applications for both in vitro fluorescence microscopy and in vivo PET imaging, novel targeted 69/71Ga/ 68Ga-protoporphyrin IX (PPIX) probes were developed. Optical analysis, fluorescence microscopy and radiolabeling with gallium-68 were performed to confirm potential applications. The use of a targeted-PPIX ring, in conjunction with 69/71Ga or 68Ga, eliminates the need for the attachment of multiple imaging tags, allowing for either in vitro fluorescent-based evaluation or in vivo nuclear-based imaging.

AB - In pursuit of a single molecule with potential applications for both in vitro fluorescence microscopy and in vivo PET imaging, novel targeted 69/71Ga/ 68Ga-protoporphyrin IX (PPIX) probes were developed. Optical analysis, fluorescence microscopy and radiolabeling with gallium-68 were performed to confirm potential applications. The use of a targeted-PPIX ring, in conjunction with 69/71Ga or 68Ga, eliminates the need for the attachment of multiple imaging tags, allowing for either in vitro fluorescent-based evaluation or in vivo nuclear-based imaging.

KW - Fluorescence microscopy

KW - Gallium-68

KW - Metal conjugates

KW - Molecular imaging

KW - PPIX

KW - Radiolabeling

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DO - 10.1016/j.apradiso.2011.11.054

M3 - Article

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SN - 0969-8043

VL - 70

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JO - Applied Radiation and Isotopes

JF - Applied Radiation and Isotopes

IS - 3

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Synthesis, radiometal labeling and in vitro evaluation of a targeted PPIX derivative

Abstract

Keywords

ASJC Scopus subject areas

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