Synthesis of thermal polymerizable alginate-GMA hydrogel for cell encapsulation

Xiaokun Wang, Tong Hao, Jing Qu, Changyong Wang, Haifeng Chen

Research output: Contribution to journalArticle

Abstract

Alginate is a negative ionic polysaccharide that is found abundantly in nature. Calcium is usually used as a cross-linker for alginate. However, calcium cross-linked alginate is used only for in vitro culture. In the present work, alginate was modified with glycidyl methacrylate (GMA) to produce a thermal polymerizable alginate-GMA (AA-GMA) macromonomer. The molecular structure and methacrylation (%DM) of the macromonomer were determined by 1H NMR. After mixing with the correct amount of initiator, the AA-GMA aqueous solution can be polymerized at physiological temperature. The AA-GMA hydrogels exhibited a three-dimensional porous structure with an average pore size ranging from 50 to 200 m, directly depending on the macromonomer concentration. Biocompatibility of the AA-GMA hydrogel was determined by in vivo muscle injection and cell encapsulation. Muscle injection in vivo showed that the AA-GMA solution mixed with initiator could form a hydrogel in situ and had a mild inflammatory effect. Human umbilical vein endothelial cells (HUVECs) were encapsulated in the AA-GMA hydrogels in situ at 37°C. Cell viability and proliferation were unaffected by macromonomer concentrations, which suggests that AA-GMA has a potential application in the field of tissue engineering, especially for myocardial repair.

Original languageEnglish (US)
Article number970619
JournalJournal of Nanomaterials
Volume2015
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Hydrogel
Alginate
Encapsulation
Hydrogels
Muscle
Calcium
Hot Temperature
alginic acid
glycidyl methacrylate
Bioelectric potentials
Endothelial cells
Polysaccharides
Biocompatibility
Tissue engineering
Molecular structure
Pore size
Repair
Cells
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Synthesis of thermal polymerizable alginate-GMA hydrogel for cell encapsulation. / Wang, Xiaokun; Hao, Tong; Qu, Jing; Wang, Changyong; Chen, Haifeng.

In: Journal of Nanomaterials, Vol. 2015, 970619, 01.01.2015.

Research output: Contribution to journalArticle

Wang, Xiaokun ; Hao, Tong ; Qu, Jing ; Wang, Changyong ; Chen, Haifeng. / Synthesis of thermal polymerizable alginate-GMA hydrogel for cell encapsulation. In: Journal of Nanomaterials. 2015 ; Vol. 2015.
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