Synthesis of PCR-derived, digoxigenin-labeled DNA probes for in situ detection of Epstein-Barr early RNAs in Epstein-Barr virus-infected cells

Sen tien Tsai, Ying tai Jin, Tzyy Choou Wu

Research output: Contribution to journalArticle

Abstract

A PCR-derived digoxigenin-labeled DNA probe was used for for Epstein-Barr early RNA (EBER) in situ hybridization in formalin-fixed paraffin-embedded tissues. The results showed that the hybridization signal was morphologically distinct and the intensity of signal was comparable with those by RNA riboprobe. The advantages of using PCR-derived DNA probes for EBER in situ hybridization include: (1) the synthesis of digoxigenin-labeled DNA probes is easy and simple by PCR; (2) the labeled amplification product can be used as a probe without further purification; (3) DNA probes are potentially more stable than RNA probes; and (4) the preparation of DNA probes is relatively efficient and rapid. It is concluded that this technique is an ideal candidate for detection of EBER expression in clinical specimens.

Original languageEnglish (US)
Pages (from-to)67-74
Number of pages8
JournalJournal of Virological Methods
Volume54
Issue number1
DOIs
StatePublished - Jul 1995

Keywords

  • DNA probe
  • Digoxigenin
  • Epstein-Barr early RNA (EBER)
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology

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