The syntheses of several tetrazolium salts are described. These include (1) an improved method for preparing the di-tetrazolium salt, Nitro-BT, (2) three iodo-5-phenyl-substituted analogs of Nitro-BT for possible use in electron microscopy and (3) five analogs of 2-P-nitrophenyl substituted monotetrazolium salts which contain 3-phenyl substituents of varying degrees of electronegativity for a study of their ability to act as electron acceptors with various dehydrogenases. Preliminary experiments with electron microscopy are given and the biochemical results with four dehydrogenases are summarized.
ASJC Scopus subject areas
- Colloid and Surface Chemistry