Synthesis of multi-walled carbon nanotubes modified with silver nanoparticles and evaluation of their antibacterial activities and cytotoxic properties

Youngmin Seo, Chanhwi Park, Jaewoo Son, Kyungwoo Lee, Jangsun Hwang, Yeonho Jo, Dohyun Lee, Muhammad Saad Khan, Sachin Ganpat Chavan, Yonghyun Choi, Dasom Kim, Assaf A. Gilad, Jonghoon Choi

Research output: Contribution to journalArticle


In this study, multi-walled carbon nanotubes (MWCNTs) were treated with an aqueous sulfuric acid solution to form an oxygen-based functional group. Silver MWCNTs were prepared by the reductive deposition of silver from an aqueous solution of AgNO3 on the oxidized MWCNTs. Given the unique color of the CNTs, it was not possible to apply them to the minimum inhibitory concentration or mitochondrial toxicity assays to evaluate the toxicity and antibacterial properties, since they would interfere with the assays. The inhibition zone and minimum bactericidal concentration for the Ag-MWCNTs were measured and Live/Dead and Trypan Blue assays were used to measure the toxicity and antibacterial properties without interfering with the color of the CNTs.

Original languageEnglish (US)
Article numbere57384
JournalJournal of Visualized Experiments
Issue number135
Publication statusPublished - May 10 2018
Externally publishedYes



  • Antibacterial property
  • Bacteria
  • Bioengineering
  • Biofilm
  • Cytotoxicity
  • Issue 135
  • Metal nanoparticle
  • Multi-walled carbon nanotube

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this