Synthesis of herpes simplex virus DNA in a soluble nuclear extract

Ricky R. Hirschhorn; Richard Abrams

doi:10.1016/0006-291X(78)91701-1

Synthesis of herpes simplex virus DNA in a soluble nuclear extract

Ricky R. Hirschhorn, Richard Abrams

School of Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A soluble extract prepared from nuclei of HeLa cells infected with herpes simplex virus type 1 has been found to synthesize herpes DNA in a process comparable to that observed in intact nuclei. The incorporation of [³H]dTTP has an absolute requirement for Mg⁺⁺ and for the other three deoxyribonucleoside triphosphates, and is relatively independent of added ATP. The reaction product, although of relatively short chain length, bands in CsCl density gradients at the density of herpes DNA and is essentially free of labeled cell DNA. Incorporation of BrdUTP results in a density shift suggesting extensive replication of endogenous DNA sequences.

Original language	English (US)
Pages (from-to)	1129-1135
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	84
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(78)91701-1
State	Published - Oct 30 1978

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(78)91701-1

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abstract = "A soluble extract prepared from nuclei of HeLa cells infected with herpes simplex virus type 1 has been found to synthesize herpes DNA in a process comparable to that observed in intact nuclei. The incorporation of [3H]dTTP has an absolute requirement for Mg++ and for the other three deoxyribonucleoside triphosphates, and is relatively independent of added ATP. The reaction product, although of relatively short chain length, bands in CsCl density gradients at the density of herpes DNA and is essentially free of labeled cell DNA. Incorporation of BrdUTP results in a density shift suggesting extensive replication of endogenous DNA sequences.",

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AU - Abrams, Richard

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AB - A soluble extract prepared from nuclei of HeLa cells infected with herpes simplex virus type 1 has been found to synthesize herpes DNA in a process comparable to that observed in intact nuclei. The incorporation of [3H]dTTP has an absolute requirement for Mg++ and for the other three deoxyribonucleoside triphosphates, and is relatively independent of added ATP. The reaction product, although of relatively short chain length, bands in CsCl density gradients at the density of herpes DNA and is essentially free of labeled cell DNA. Incorporation of BrdUTP results in a density shift suggesting extensive replication of endogenous DNA sequences.

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Synthesis of herpes simplex virus DNA in a soluble nuclear extract

Abstract

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