Synthesis of herpes simplex virus DNA in a soluble nuclear extract

Ricky R. Hirschhorn, Richard Abrams

Research output: Contribution to journalArticle

Abstract

A soluble extract prepared from nuclei of HeLa cells infected with herpes simplex virus type 1 has been found to synthesize herpes DNA in a process comparable to that observed in intact nuclei. The incorporation of [3H]dTTP has an absolute requirement for Mg++ and for the other three deoxyribonucleoside triphosphates, and is relatively independent of added ATP. The reaction product, although of relatively short chain length, bands in CsCl density gradients at the density of herpes DNA and is essentially free of labeled cell DNA. Incorporation of BrdUTP results in a density shift suggesting extensive replication of endogenous DNA sequences.

Original languageEnglish (US)
Pages (from-to)1129-1135
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume84
Issue number4
DOIs
StatePublished - Oct 30 1978
Externally publishedYes

Fingerprint

Simplexvirus
Viruses
DNA
Deoxyribonucleosides
DNA sequences
Human Herpesvirus 1
Reaction products
Chain length
HeLa Cells
Adenosine Triphosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synthesis of herpes simplex virus DNA in a soluble nuclear extract. / Hirschhorn, Ricky R.; Abrams, Richard.

In: Biochemical and Biophysical Research Communications, Vol. 84, No. 4, 30.10.1978, p. 1129-1135.

Research output: Contribution to journalArticle

Hirschhorn, Ricky R. ; Abrams, Richard. / Synthesis of herpes simplex virus DNA in a soluble nuclear extract. In: Biochemical and Biophysical Research Communications. 1978 ; Vol. 84, No. 4. pp. 1129-1135.
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