Synthesis of Chromogenic Arginine Derivatives as Substrates for Trypsin

Robert E. Plapinger, Marvin M. Nachlas, Myron L. Seligman, Arnold M. Seligman

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The synthesis of 15 chromogenic substrates which are split rapidly by trypsin is described. All are derivatives of arginine and were prepared by the mixed anhydride method utilizing the protonation blocking procedure for masking the guanidine group. The three substrates most rapidly hydrolyzed are N-carbobenzoxyglycyl-glycyl-L-arginine-2-naphthylamide hydrochloride, β-carboxyproprionyl-L-arginyl-L-arginine-2-naphthylamide dihydrochloride, and N-carbobenzoxy-L-arginyl-L-arginyl-L-arginine-2-naphthylamide trihydrochloride.

Original languageEnglish (US)
Pages (from-to)1781-1785
Number of pages5
JournalJournal of Organic Chemistry
Issue number6
StatePublished - Jan 1 1965

ASJC Scopus subject areas

  • Organic Chemistry


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