Synthesis of a collagenase inhibitor by smooth muscle cells in culture

Joseph C. Nolan, Susan Ridge, Arnold L. Oronsky, Linda L. Slakey, S. S. Kerwar

Research output: Contribution to journalArticle

Abstract

Serum-free medium that was incubated for 24 hours with confluent cultures of smooth muscle cells contains a potent inhibitor of mammalian collagenase but not of bacterial collagenase. Synthesis of this inhibitor by these cultures is blocked by cycloheximide. The inhibitor is sensitive to trypsin but is stable to heating at 60° for 10 minutes and to acid treatment. Reduction with mercaptoethanol followed by alkylation destroys the inhibitory activity. This inhibitor may play a physiological role in the slow turnover of collagen that is observed in the vasculature.

Original languageEnglish (US)
Pages (from-to)1183-1190
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume83
Issue number3
DOIs
StatePublished - Aug 14 1978
Externally publishedYes

Fingerprint

Matrix Metalloproteinase Inhibitors
Mercaptoethanol
Serum-Free Culture Media
Alkylation
Collagenases
Cycloheximide
Cell culture
Heating
Trypsin
Smooth Muscle Myocytes
Muscle
Collagen
Cell Culture Techniques
Cells
Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Synthesis of a collagenase inhibitor by smooth muscle cells in culture. / Nolan, Joseph C.; Ridge, Susan; Oronsky, Arnold L.; Slakey, Linda L.; Kerwar, S. S.

In: Biochemical and Biophysical Research Communications, Vol. 83, No. 3, 14.08.1978, p. 1183-1190.

Research output: Contribution to journalArticle

Nolan, Joseph C. ; Ridge, Susan ; Oronsky, Arnold L. ; Slakey, Linda L. ; Kerwar, S. S. / Synthesis of a collagenase inhibitor by smooth muscle cells in culture. In: Biochemical and Biophysical Research Communications. 1978 ; Vol. 83, No. 3. pp. 1183-1190.
@article{dd94528ec23545489b76a870358f8c60,
title = "Synthesis of a collagenase inhibitor by smooth muscle cells in culture",
abstract = "Serum-free medium that was incubated for 24 hours with confluent cultures of smooth muscle cells contains a potent inhibitor of mammalian collagenase but not of bacterial collagenase. Synthesis of this inhibitor by these cultures is blocked by cycloheximide. The inhibitor is sensitive to trypsin but is stable to heating at 60° for 10 minutes and to acid treatment. Reduction with mercaptoethanol followed by alkylation destroys the inhibitory activity. This inhibitor may play a physiological role in the slow turnover of collagen that is observed in the vasculature.",
author = "Nolan, {Joseph C.} and Susan Ridge and Oronsky, {Arnold L.} and Slakey, {Linda L.} and Kerwar, {S. S.}",
year = "1978",
month = "8",
day = "14",
doi = "10.1016/0006-291X(78)91520-6",
language = "English (US)",
volume = "83",
pages = "1183--1190",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Synthesis of a collagenase inhibitor by smooth muscle cells in culture

AU - Nolan, Joseph C.

AU - Ridge, Susan

AU - Oronsky, Arnold L.

AU - Slakey, Linda L.

AU - Kerwar, S. S.

PY - 1978/8/14

Y1 - 1978/8/14

N2 - Serum-free medium that was incubated for 24 hours with confluent cultures of smooth muscle cells contains a potent inhibitor of mammalian collagenase but not of bacterial collagenase. Synthesis of this inhibitor by these cultures is blocked by cycloheximide. The inhibitor is sensitive to trypsin but is stable to heating at 60° for 10 minutes and to acid treatment. Reduction with mercaptoethanol followed by alkylation destroys the inhibitory activity. This inhibitor may play a physiological role in the slow turnover of collagen that is observed in the vasculature.

AB - Serum-free medium that was incubated for 24 hours with confluent cultures of smooth muscle cells contains a potent inhibitor of mammalian collagenase but not of bacterial collagenase. Synthesis of this inhibitor by these cultures is blocked by cycloheximide. The inhibitor is sensitive to trypsin but is stable to heating at 60° for 10 minutes and to acid treatment. Reduction with mercaptoethanol followed by alkylation destroys the inhibitory activity. This inhibitor may play a physiological role in the slow turnover of collagen that is observed in the vasculature.

UR - http://www.scopus.com/inward/record.url?scp=0017821447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017821447&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(78)91520-6

DO - 10.1016/0006-291X(78)91520-6

M3 - Article

C2 - 213065

AN - SCOPUS:0017821447

VL - 83

SP - 1183

EP - 1190

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -