Serum-free medium that was incubated for 24 hours with confluent cultures of smooth muscle cells contains a potent inhibitor of mammalian collagenase but not of bacterial collagenase. Synthesis of this inhibitor by these cultures is blocked by cycloheximide. The inhibitor is sensitive to trypsin but is stable to heating at 60° for 10 minutes and to acid treatment. Reduction with mercaptoethanol followed by alkylation destroys the inhibitory activity. This inhibitor may play a physiological role in the slow turnover of collagen that is observed in the vasculature.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 14 1978|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology