Synthesis of a collagenase inhibitor by smooth muscle cells in culture

Joseph C. Nolan, Susan Ridge, Arnold L. Oronsky, Linda L. Slakey, S. S. Kerwar

Research output: Contribution to journalArticle

Abstract

Serum-free medium that was incubated for 24 hours with confluent cultures of smooth muscle cells contains a potent inhibitor of mammalian collagenase but not of bacterial collagenase. Synthesis of this inhibitor by these cultures is blocked by cycloheximide. The inhibitor is sensitive to trypsin but is stable to heating at 60° for 10 minutes and to acid treatment. Reduction with mercaptoethanol followed by alkylation destroys the inhibitory activity. This inhibitor may play a physiological role in the slow turnover of collagen that is observed in the vasculature.

Original languageEnglish (US)
Pages (from-to)1183-1190
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume83
Issue number3
DOIs
StatePublished - Aug 14 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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