Synthesis, characterization, and self-assembly with plasmid DNA of a quaternary ammonium derivative of pectic galactan and its fluorescent labeling for bioimaging applications

Ramesh Chintakunta, Nitsa Buaron, Nicole Kahn, Amana Moriah, Rinat Lifshiz, Riki Goldbart, Tamar Traitel, Betty Tyler, Henry Brem, Joseph Kost

Research output: Contribution to journalArticlepeer-review

Abstract

Quaternized derivatives of pectic galactan (QPG) were synthesized by a reaction of pectic galactan (PG) with 3-chloro-2-hydroxypropyl trimethyl ammonium chloride (CHPTAC) in the presence of aqueous sodium hydroxide solution under mild reaction conditions. The results showed that the concentration of CHPTAC and NaOH has great impact on the quaternization reaction. QPG was found to interact electrostatically with plasmid DNA in aqueous solution to form complexes in globular condensed morphology in a nanometer scale size ranging from 60 to 160 nm. Complexes formed with QPG fluorescently labeled with 5-DTAF (QPG-5-DTAF) were introduced to the C6 rat glioma cell line, and were found to be able to enter the cell and approach the nucleus within 24 h. The results suggest that this type of modified natural polysaccharide may have an advantage as a biocompatible and biodegradable gene delivery carrier and furthermore may serve as a cell specific carrier.

Original languageEnglish (US)
Pages (from-to)308-318
Number of pages11
JournalCarbohydrate Polymers
Volume150
DOIs
StatePublished - Oct 5 2016

Keywords

  • 5-DTAF
  • C6 rat glioma cells
  • CHPTAC
  • Cell specific carrier
  • Pectic galactan
  • Quaternized pectic galactan

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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