The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.
|Translated title of the contribution||Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells|
|Number of pages||5|
|Journal||Molekuliarnaia genetika, mikrobiologiia i virusologiia|
|State||Published - May 1990|
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