Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.

Translated title of the contribution: Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells

E. A. Ratovitskiǐ, A. B. Rudenko, N. A. Vasinova, O. V. Kidgotko, B. K. Nisman

Research output: Contribution to journalArticle

Abstract

The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

Original languageRussian
Pages (from-to)20-24
Number of pages5
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number5
StatePublished - May 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

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