Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.

Translated title of the contribution: Synthesis and secretion of recombinant protein--the product of v-sis oncogene in Saccharomyces cerevisiae cells

E. A. Ratovitskiǐ, A. B. Rudenko, N. A. Vasinova, O. V. Kidgotko, B. K. Nisman

Research output: Contribution to journalArticle

Abstract

The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

Original languageRussian
Pages (from-to)20-24
Number of pages5
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number5
StatePublished - May 1990
Externally publishedYes

Fingerprint

Oncogene Proteins v-sis
Recombinant Proteins
Saccharomyces cerevisiae
Plasmids
Recombinant DNA
Galactose
Immunoblotting
Electrophoresis
Cell Culture Techniques
Gels
Cell Proliferation
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae. / Ratovitskiǐ, E. A.; Rudenko, A. B.; Vasinova, N. A.; Kidgotko, O. V.; Nisman, B. K.

In: Molekuliarnaia genetika, mikrobiologiia i virusologiia, No. 5, 05.1990, p. 20-24.

Research output: Contribution to journalArticle

Ratovitskiǐ, E. A. ; Rudenko, A. B. ; Vasinova, N. A. ; Kidgotko, O. V. ; Nisman, B. K. / Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae. In: Molekuliarnaia genetika, mikrobiologiia i virusologiia. 1990 ; No. 5. pp. 20-24.
@article{83bc273c9d194d28bd7c0b483e951194,
title = "Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.",
abstract = "The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.",
author = "Ratovitskiǐ, {E. A.} and Rudenko, {A. B.} and Vasinova, {N. A.} and Kidgotko, {O. V.} and Nisman, {B. K.}",
year = "1990",
month = "5",
language = "Russian",
pages = "20--24",
journal = "Molekuliarnaia genetika, mikrobiologiia i virusologiia",
issn = "0208-0613",
publisher = "Meditsina Publishers",
number = "5",

}

TY - JOUR

T1 - Sintez i sekretsiia rekombinantnogo belka--produkta onkogena v-sis v kletkakh drozhzheǐ Saccharomyces cerevisiae.

AU - Ratovitskiǐ, E. A.

AU - Rudenko, A. B.

AU - Vasinova, N. A.

AU - Kidgotko, O. V.

AU - Nisman, B. K.

PY - 1990/5

Y1 - 1990/5

N2 - The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

AB - The recombinant plasmid DNA YEp secl-v-sis was constructed. This plasmid was able to code for the synthesis and secretion into the cultural medium of the protein-product of oncogene v-sis. Transcription, copy number and stability of the plasmid DNA were studied under the conditions of galactose induction. The v-sis protein was determined by gel electrophoresis and immunoblotting methods and assayed for cell-proliferation activity in the mammalian cell culture.

UR - http://www.scopus.com/inward/record.url?scp=0025426994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025426994&partnerID=8YFLogxK

M3 - Article

C2 - 2199826

AN - SCOPUS:0025426994

SP - 20

EP - 24

JO - Molekuliarnaia genetika, mikrobiologiia i virusologiia

JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia

SN - 0208-0613

IS - 5

ER -