Synthesis and peptide incorporation of an unnatural amino acid containing activity-based probe for protein tyrosine phosphatases

Kui Shen, Lixin Qi, Mohini Ravula, Krzysztof Klimaszewski

Research output: Contribution to journalArticle

Abstract

An unnatural amino acid was synthesized to incorporate a quinone methide-generating activity-based probe for protein tyrosine phosphatases (PTPs) and then integrated into a PTP1B-specific substrate. The resulting probe led to preferential labeling of PTP1B in cell lysates in the presence of PTP4A3.

Original languageEnglish (US)
Pages (from-to)3264-3267
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume19
Issue number12
DOIs
StatePublished - Jun 15 2009
Externally publishedYes

Keywords

  • Activity-based probe
  • Affinity labeling
  • Protein tyrosine phosphatase
  • PTP1B
  • PTP4A3
  • Quinone methide
  • Unnatural amino acid

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

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