Synthesis and characterization of a novel degradable phosphate-containing hydrogel

Dong An Wang, Christopher G. Williams, Qiang Li, Blanka Sharma, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

A phosphate-containing and photocrosslinkable polymer, poly(ethylene glycol) di-[ethyl phosphatidyl (ethylene glycol) methacrylate], "PhosPEG-dMA", was synthesized. As a water-soluble macromer, PhosPEG-dMA is suitable for in situ injection and cell-encapsulation by light-induced gelation to produce a novel biocompatible and biodegradable hydrogel for application to cartilage and bone tissue engineering. 1H-NMR, MALDI-TOF mass spectrometry, and elemental analysis were performed to characterize the macromer. Fifteen and 20% (w/v) PhosPEG gels were photopolymerized using UV light with 0.05% photoinitiator. The swelling and water content of the hydrogels was studied and the crosslinking efficiency (density) of the macromers was simulated based on the Peppas-Merrill model. Torsional mechanical analysis of the gels demonstrated a viscoelastic characteristic with high elasticity. The results indicated that, with the fixed PEG-segment size, the greater strength and water-content of the gels depend on the higher crosslinking density. Degradation experiments revealed a linear dry-weight loss of 22.88% and 16.08% from 15% and 20% PhosPEG gels after 9 weeks. The 31P-NMR detected the signals of both phosphate and phosphoric acid in the degrading systems (the gel bulks and the supernatants). Finally, human mesenchymal stem cells (hMSC) were encapsulated into PhosPEG Gel constructs and remained viable as qualitatively demonstrated by "Live/Dead" cell staining assay and MTT assay. The cell-encapsulation efficiency was determined by the characterization of DNA content in each gel construct and the semi-quantitative analysis of the cell viability was also performed by the DNA assay combined with MTT assay.

Original languageEnglish (US)
Pages (from-to)3969-3980
Number of pages12
JournalBiomaterials
Volume24
Issue number22
DOIs
StatePublished - Oct 2003

Fingerprint

Hydrogel
Hydrogels
Phosphates
Gels
Assays
Ethylene Glycol
Encapsulation
Crosslinking
Water content
Polyethylene glycols
Water
DNA
Nuclear magnetic resonance
Methacrylates
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Elasticity
Cartilage
Phosphoric acid
Gelation
Tissue Engineering

Keywords

  • Degradation
  • Hydrogel
  • Macromer
  • Phosphate
  • Photopolymerization
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Synthesis and characterization of a novel degradable phosphate-containing hydrogel. / Wang, Dong An; Williams, Christopher G.; Li, Qiang; Sharma, Blanka; Elisseeff, Jennifer Hartt.

In: Biomaterials, Vol. 24, No. 22, 10.2003, p. 3969-3980.

Research output: Contribution to journalArticle

Wang, Dong An ; Williams, Christopher G. ; Li, Qiang ; Sharma, Blanka ; Elisseeff, Jennifer Hartt. / Synthesis and characterization of a novel degradable phosphate-containing hydrogel. In: Biomaterials. 2003 ; Vol. 24, No. 22. pp. 3969-3980.
@article{4bb1d8914f9343fab732854a74d337f7,
title = "Synthesis and characterization of a novel degradable phosphate-containing hydrogel",
abstract = "A phosphate-containing and photocrosslinkable polymer, poly(ethylene glycol) di-[ethyl phosphatidyl (ethylene glycol) methacrylate], {"}PhosPEG-dMA{"}, was synthesized. As a water-soluble macromer, PhosPEG-dMA is suitable for in situ injection and cell-encapsulation by light-induced gelation to produce a novel biocompatible and biodegradable hydrogel for application to cartilage and bone tissue engineering. 1H-NMR, MALDI-TOF mass spectrometry, and elemental analysis were performed to characterize the macromer. Fifteen and 20{\%} (w/v) PhosPEG gels were photopolymerized using UV light with 0.05{\%} photoinitiator. The swelling and water content of the hydrogels was studied and the crosslinking efficiency (density) of the macromers was simulated based on the Peppas-Merrill model. Torsional mechanical analysis of the gels demonstrated a viscoelastic characteristic with high elasticity. The results indicated that, with the fixed PEG-segment size, the greater strength and water-content of the gels depend on the higher crosslinking density. Degradation experiments revealed a linear dry-weight loss of 22.88{\%} and 16.08{\%} from 15{\%} and 20{\%} PhosPEG gels after 9 weeks. The 31P-NMR detected the signals of both phosphate and phosphoric acid in the degrading systems (the gel bulks and the supernatants). Finally, human mesenchymal stem cells (hMSC) were encapsulated into PhosPEG Gel constructs and remained viable as qualitatively demonstrated by {"}Live/Dead{"} cell staining assay and MTT assay. The cell-encapsulation efficiency was determined by the characterization of DNA content in each gel construct and the semi-quantitative analysis of the cell viability was also performed by the DNA assay combined with MTT assay.",
keywords = "Degradation, Hydrogel, Macromer, Phosphate, Photopolymerization, Tissue engineering",
author = "Wang, {Dong An} and Williams, {Christopher G.} and Qiang Li and Blanka Sharma and Elisseeff, {Jennifer Hartt}",
year = "2003",
month = "10",
doi = "10.1016/S0142-9612(03)00280-1",
language = "English (US)",
volume = "24",
pages = "3969--3980",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "22",

}

TY - JOUR

T1 - Synthesis and characterization of a novel degradable phosphate-containing hydrogel

AU - Wang, Dong An

AU - Williams, Christopher G.

AU - Li, Qiang

AU - Sharma, Blanka

AU - Elisseeff, Jennifer Hartt

PY - 2003/10

Y1 - 2003/10

N2 - A phosphate-containing and photocrosslinkable polymer, poly(ethylene glycol) di-[ethyl phosphatidyl (ethylene glycol) methacrylate], "PhosPEG-dMA", was synthesized. As a water-soluble macromer, PhosPEG-dMA is suitable for in situ injection and cell-encapsulation by light-induced gelation to produce a novel biocompatible and biodegradable hydrogel for application to cartilage and bone tissue engineering. 1H-NMR, MALDI-TOF mass spectrometry, and elemental analysis were performed to characterize the macromer. Fifteen and 20% (w/v) PhosPEG gels were photopolymerized using UV light with 0.05% photoinitiator. The swelling and water content of the hydrogels was studied and the crosslinking efficiency (density) of the macromers was simulated based on the Peppas-Merrill model. Torsional mechanical analysis of the gels demonstrated a viscoelastic characteristic with high elasticity. The results indicated that, with the fixed PEG-segment size, the greater strength and water-content of the gels depend on the higher crosslinking density. Degradation experiments revealed a linear dry-weight loss of 22.88% and 16.08% from 15% and 20% PhosPEG gels after 9 weeks. The 31P-NMR detected the signals of both phosphate and phosphoric acid in the degrading systems (the gel bulks and the supernatants). Finally, human mesenchymal stem cells (hMSC) were encapsulated into PhosPEG Gel constructs and remained viable as qualitatively demonstrated by "Live/Dead" cell staining assay and MTT assay. The cell-encapsulation efficiency was determined by the characterization of DNA content in each gel construct and the semi-quantitative analysis of the cell viability was also performed by the DNA assay combined with MTT assay.

AB - A phosphate-containing and photocrosslinkable polymer, poly(ethylene glycol) di-[ethyl phosphatidyl (ethylene glycol) methacrylate], "PhosPEG-dMA", was synthesized. As a water-soluble macromer, PhosPEG-dMA is suitable for in situ injection and cell-encapsulation by light-induced gelation to produce a novel biocompatible and biodegradable hydrogel for application to cartilage and bone tissue engineering. 1H-NMR, MALDI-TOF mass spectrometry, and elemental analysis were performed to characterize the macromer. Fifteen and 20% (w/v) PhosPEG gels were photopolymerized using UV light with 0.05% photoinitiator. The swelling and water content of the hydrogels was studied and the crosslinking efficiency (density) of the macromers was simulated based on the Peppas-Merrill model. Torsional mechanical analysis of the gels demonstrated a viscoelastic characteristic with high elasticity. The results indicated that, with the fixed PEG-segment size, the greater strength and water-content of the gels depend on the higher crosslinking density. Degradation experiments revealed a linear dry-weight loss of 22.88% and 16.08% from 15% and 20% PhosPEG gels after 9 weeks. The 31P-NMR detected the signals of both phosphate and phosphoric acid in the degrading systems (the gel bulks and the supernatants). Finally, human mesenchymal stem cells (hMSC) were encapsulated into PhosPEG Gel constructs and remained viable as qualitatively demonstrated by "Live/Dead" cell staining assay and MTT assay. The cell-encapsulation efficiency was determined by the characterization of DNA content in each gel construct and the semi-quantitative analysis of the cell viability was also performed by the DNA assay combined with MTT assay.

KW - Degradation

KW - Hydrogel

KW - Macromer

KW - Phosphate

KW - Photopolymerization

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=0038354754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038354754&partnerID=8YFLogxK

U2 - 10.1016/S0142-9612(03)00280-1

DO - 10.1016/S0142-9612(03)00280-1

M3 - Article

C2 - 12834592

AN - SCOPUS:0038354754

VL - 24

SP - 3969

EP - 3980

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 22

ER -