Syntheses of DNA duplexes that contain a N 4C-Alkyl-NN 4CC Interstrand cross-link

Paul S. Miller

Research output: Contribution to journalArticle

Abstract

This unit describes a simple procedure for preparing short DNA duplexes that contain a singleNN 4CC-alkyl-NN 4CC interstrand cross-link. The synthesis is carried out on an automated DNAsynthesizer using standard phosphoramidite chemistry. The cross-link is introduced during the synthesis of the duplex. Themethod can be used to prepare milligram quantities of cross-linked duplexes suitable for physical studies and for the preparation of larger DNA molecules that can be used as substrates to study DNA repair in whole cell extracts and in living cells in culture.

Original languageEnglish (US)
JournalCurrent Protocols in Nucleic Acid Chemistry
Issue numberSUPPL.44
DOIs
StatePublished - Aug 29 2011

Keywords

  • DNA duplex
  • Interstrand cross-link
  • Oligonucleotide
  • Synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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