Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDITOF mass spectrometry and/or by enzymatic digestion.
ASJC Scopus subject areas
- Molecular Medicine