Syntheses of DNA duplexes containing a C-C interstrand cross-link

Anne M. Noronha, David M. Noll, Paul S. Miller

Research output: Contribution to journalArticlepeer-review

Abstract

Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDITOF mass spectrometry and/or by enzymatic digestion.

Original languageEnglish (US)
Pages (from-to)1303-1307
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume20
Issue number4-7
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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