Syntheses of DNA duplexes containing a C-C interstrand cross-link

Anne M. Noronha; David M. Noll; Paul S. Miller

doi:10.1081/NCN-100002542

Syntheses of DNA duplexes containing a C-C interstrand cross-link

Anne M. Noronha, David M. Noll, Paul S. Miller

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Short DNA duplexes that contain a N⁴C-ethyl-N⁴C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDITOF mass spectrometry and/or by enzymatic digestion.

Original language	English (US)
Pages (from-to)	1303-1307
Number of pages	5
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	20
Issue number	4-7
DOIs	https://doi.org/10.1081/NCN-100002542
State	Published - 2001
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Genetics

Access to Document

10.1081/NCN-100002542

Cite this

@article{29cb72a18f474fbbb5aebec4ccb07938,

title = "Syntheses of DNA duplexes containing a C-C interstrand cross-link",

abstract = "Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDITOF mass spectrometry and/or by enzymatic digestion.",

author = "Noronha, {Anne M.} and Noll, {David M.} and Miller, {Paul S.}",

year = "2001",

doi = "10.1081/NCN-100002542",

language = "English (US)",

volume = "20",

pages = "1303--1307",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

issn = "1525-7770",

publisher = "Taylor and Francis Ltd.",

number = "4-7",

}

TY - JOUR

T1 - Syntheses of DNA duplexes containing a C-C interstrand cross-link

AU - Noronha, Anne M.

AU - Noll, David M.

AU - Miller, Paul S.

PY - 2001

Y1 - 2001

N2 - Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDITOF mass spectrometry and/or by enzymatic digestion.

AB - Short DNA duplexes that contain a N4C-ethyl-N4C interstrand cross-link were prepared on controlled pore glass supports using a DNA synthesizer. The C-C cross-link was introduced via a convertible nucleoside on the support or by using a protected C-C cross-link phosphoramidite. An orthogonal protection scheme allowed selective chain growth in either a 3′ → 5′ or 5′ → 3′ direction. The cross-linked duplexes were purified by HPLC and characterized by MALDITOF mass spectrometry and/or by enzymatic digestion.

UR - http://www.scopus.com/inward/record.url?scp=0034839706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034839706&partnerID=8YFLogxK

U2 - 10.1081/NCN-100002542

DO - 10.1081/NCN-100002542

M3 - Article

C2 - 11563009

AN - SCOPUS:0034839706

SN - 1525-7770

VL - 20

SP - 1303

EP - 1307

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 4-7

ER -

Syntheses of DNA duplexes containing a C-C interstrand cross-link

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this