Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo

Mark Wu, Tim Fergestad, Thomas Lloyd, Yuchun He, Kendal Broadie, Hugo J. Bellen

Research output: Contribution to journalArticle

Abstract

Biochemical studies suggest that syntaxin 1A participates in multiple protein-protein interactions in the synaptic terminal, but the in vivo significance of these interactions is poorly understood. We used a targeted mutagenesis approach to eliminate specific syntaxin binding interactions and demonstrate that Drosophila syntaxin 1A plays multiple regulatory roles in neurotransmission in vivo. Syntaxin mutations that eliminate ROP/Munc-18 binding display increased neurotransmitter release, suggesting that ROP inhibits neurosecretion through its interaction with syntaxin. Syntaxin mutations that block Ca2+ channel binding also cause an increase in neurotransmitter release, suggesting that syntaxin normally functions in inhibiting Ca2+ channel opening. Additionally, we identify and characterize a syntaxin Ca2+ effector domain, which may spatially organize the Ca2+ channel, cysteine string protein, and synaptotagmin for effective excitation- secretion coupling in the presynaptic terminal.

Original languageEnglish (US)
Pages (from-to)593-605
Number of pages13
JournalNeuron
Volume23
Issue number3
DOIs
StatePublished - Jul 1999
Externally publishedYes

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Syntaxin 1
Qa-SNARE Proteins
Neurotransmitter Agents
Proteins
Presynaptic Terminals
Synaptotagmins
Neurosecretion
Mutation
Synaptic Transmission
Mutagenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo. / Wu, Mark; Fergestad, Tim; Lloyd, Thomas; He, Yuchun; Broadie, Kendal; Bellen, Hugo J.

In: Neuron, Vol. 23, No. 3, 07.1999, p. 593-605.

Research output: Contribution to journalArticle

Wu, Mark ; Fergestad, Tim ; Lloyd, Thomas ; He, Yuchun ; Broadie, Kendal ; Bellen, Hugo J. / Syntaxin 1A interacts with multiple exocytic proteins to regulate neurotransmitter release in vivo. In: Neuron. 1999 ; Vol. 23, No. 3. pp. 593-605.
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