SYNPLA, a method to identify synapses displaying plasticity after learning

Kim Dore, Yvonne Pao, Jose Soria Lopez, Sage Aronson, Huiqing Zhan, Sanchari Ghosh, Sabina Merrill, Anthony M. Zador, Roberto Malinow, Justus M. Kebschull

Research output: Contribution to journalArticlepeer-review

Abstract

Which neural circuits undergo synaptic changes when an animal learns? Although it is widely accepted that changes in synaptic strength underlie many forms of learning and memory, it remains challenging to connect changes in synaptic strength at specific neural pathways to specific behaviors and memories. Here we introduce SYNPLA (synaptic proximity ligation assay), a synapse-specific, high-throughput, and potentially brain-wide method capable of detecting circuit-specific learning-induced synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)3214-3219
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number6
DOIs
StatePublished - Feb 11 2020
Externally publishedYes

Keywords

  • Defense conditioning
  • Fear conditioning
  • GluA1
  • Proximity ligation assay
  • Synaptic potentiation

ASJC Scopus subject areas

  • General

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